缓慢性心律失常大鼠针刺血清对心肌细胞内Ca^(2+)浓度影响的研究  

Study of Influence of Acupuncture on Myocardial Intracellular Ca^(2+) Concentration in Serum of Rat with Slow Arrhythmia

作　　者：张小卿[1] 韩涛[2] 马贤德[1] 荆秦[1] 田国伟[1] 

机构地区：[1]辽宁中医药大学针灸推拿学院,辽宁沈阳110847 [2]沈阳市骨科医院,辽宁沈阳110044

出　　处：《辽宁中医杂志》2013年第11期2379-2381,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：辽宁省教育厅科学技术研究项目(L2010350)

摘　　要：目的:观察缓慢性心律失常大鼠针刺血清对乳鼠心肌细胞内Ca2+浓度的影响,以探讨针刺治疗心律失常的离子通道机制。方法:取新生SD大鼠心肌细胞进行原代培养,5 d后应用荧光分子探针Fluo-3AM染色,应用激光共聚焦技术观察乳鼠心肌细胞加入不同类型血清后Ca2+浓度变化情况。血清制备方法是将SD大鼠随机分为正常组、模型组(注射维拉帕米造模)、针刺组(维拉帕米模型加电针"内关"穴)。结果:与空白对照组相比,模型血清组和针刺血清组Ca2+平均荧光强度值均降低,差异有统计学意义(P<0.01);与模型血清组相比,针刺血清组Ca2+平均荧光强度值升高,差异有统计学意义(P<0.05)。结论:针刺可以治疗大鼠缓慢性心律失常,其机制为针刺血清中有某种活性物质可以升高维拉帕米致心律失常大鼠心肌细胞内Ca2+浓度。Objective：To observe the effect of acupuncture serum on the myocardial intracellular Ca^2＋ concentration in rats with slow arrhythmia and explore the mechanism of acupuncture treatment of arrhythmia ion channel. Methods ： The newborn SD rat myocardial ceils were taken to culture the original generation. Five days later, the fluorescent molecular probes were used for Fluo -3AM staining. The confocal laser technology application was to observe the Ca^2＋ concentration changes of rat myocardial cells adding different kinds of serum. Serum preparation method was to randomly divide SD rats into normal group, model group （ built by injection of verapamil）, acupuncture group （Neiguan with electroacupuncture）. Results：Compared with blank control group, model and acupuncture serum group average fluorescence intensity of Ca^2＋ reduced, the difference was statistically significant （ P 〈 0. 01 ）. Compared with the model serum group, acupuncture serum group average fluorescence intensity of Ca^2＋ increased, the difference was statistically significant （P 〈 0. 05 ）. Conclusion ： Acupuncture can treat slow arrhythmia in rats ; the mechanism is that acupuncture serum has certain active substances which can increase Ca^2 ＋ concentration of verapamil that cause arrhythmia in rats myocardial cells.

关 键 词：心律失常 钙离子 针刺血清 

分 类 号：R541.7[医药卫生—心血管疾病]