干旱胁迫对扁穗牛鞭草叶片抗氧化系统及膜脂过氧化的影响  

Effects of Drought Stress on Leaf Antioxidant System and Membrane Lipid Peroxidation of Hemarthria compressa

作　　者：杨盛婷 黄琳凯[1] 张新全[1] 蒋晓梅[1] 严海东[1] 黄秀[1] 

机构地区：[1]四川农业大学草业科学系,四川雅安625014

出　　处：《热带作物学报》2013年第11期2083-2089,共7页Chinese Journal of Tropical Crops

基　　金：四川省育种攻关(No.2011YZGG-11);国家现代牧草产业技术体(No.CARS-35-05);教育部春晖计划;四川农业大学大学生科研兴趣计划

摘　　要：通过室内盆栽试验,以抗旱能力不同的H036、H042、广益、2003-5扁穗牛鞭草为材料,定期测定土壤含水量、叶片相对含水量、丙二醛(MDA)、电导率、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)等生理指标,研究干旱胁迫下扁穗牛鞭草叶片膜脂过氧化作用、非酶类保护物质含量和保护酶活性的生理变化情况,探讨扁穗牛鞭草材料之间抗旱机制的差异。结果表明:在干旱胁迫期间,扁穗牛鞭草叶片的MDA含量升高,而两种有关活性氧清除的物质CAT和SOD活性先增强后减弱,而非酶类抗氧化物质GSH含量呈现下降趋势。与抗旱性弱的扁穗牛鞭草材料相比,抗旱性强的材料,酶活性相对较高,丙二醛含量较低,在恢复正常供水后,能较快达到正常水平。综上所述,抗旱性强的扁穗牛鞭草材料在干旱胁迫下酶类和非酶类抗氧化保护系统比抗旱性弱的强。A green house pot experiment was carried out to regularly determine soil water content,leaf relative water content,malondialdehyde （MDA）,conductance,superoxide dismutase （SOD）,catalase （CAT） and glutathione using four varieties including ‘H036’,‘H042’,‘Guangyi’ and ‘2003-5’ of Hemarthria compressa with different drought resistance.This article was to study the changes of membrane lipid peroxidation,non-enzyme protective substances and protective enzyme activity and discuss the differences of drought resistance mechanism in the materials of Hemarthria compressa.The results indicated that the MDA,CAT and SOD content increased,after that,the content of CAT and SOD declined and the non-enzymes antioxidants （GSH） decreased during drought stress.The materials with high drought resistance had higher enzyme activity,lower MDA and could recover to normal physiology biochemistry activity faster after rewatering.In general,there were higher endogenous coordination of antioxidation enzyme system and non-enzyme system in strong drought resistant Hemarthria compressa.

关 键 词：扁穗牛鞭草 干旱胁迫 抗氧化 膜脂过氧化 抗旱性 

分 类 号：S543.9[农业科学—作物学]