正红菇、牛肝菌外生菌根内层形态和结构的扫描电镜观察  

The Observation of Mycorrhizal Microstructure with Scanning Elcetron Microscope(SEM)

作　　者：黄敏敏[1,2] 陈宇航[3] 姚清华[1,2] 

机构地区：[1]福建省农业科学院中心实验室,福建福州350003 [2]福建省精密仪器农业测试重点实验室,福建福州350003 [3]福建省农业科学院食用菌研究所,福建福州350003

出　　处：《热带作物学报》2013年第11期2097-2101,共5页Chinese Journal of Tropical Crops

基　　金：福建省农业科学院青年创新基金(No.2011QB-18)

摘　　要：对壳斗科树（与正红菇、牛肝菌共生）共生菌根的内层超微结构进行扫描电镜观察前处理的研究,以对上述菌根及与其相关的菌根菌的识别方法进行补充.结果表明,进行菌根内层超微结构的扫描电镜观察,应选择浅棕色、直径1～2 mm的成熟细根部分.通过不同浓度氢氧化钙、不同时间的处理,可获得清晰的可识别的哈蒂氏网或类似图像.菌根的哈蒂氏网形态结构具有一定的稳定性.This paper is to explore the observation condition of mycorrhizal microstructure with SEM and observe them in order to make a supplement of identification methods to mycorrhiza and mycorrhizal fungi.The results showed that the light brown and mature fine root with 1～2 mm diameter should be selected.The microtubule and hartig net images could be obtained clearly and identifiably by being treated with different Ca（OH）2 concentrations and times.The structure of microtubule was certain stability and could be used to identify mycorrhiza and mycorrhizal fungi.

关 键 词：菌根 哈蒂氏网 扫描电镜观察 

分 类 号：S646[农业科学—蔬菜学]