与甘蔗抗黑穗病基因连锁的SSR标记筛选  

Screening of SSR Marker Linked to Smut Resistance Gene in Sugarcane

作　　者：高轶静[1] 张荣华[1] 张革民[1] 杨柳[1] 段维兴[1] 王泽平[1] 杨翠芳[1] 游建华[1] 

机构地区：[1]广西农业科学院甘蔗研究所中国农业科学院甘蔗研究中心广西甘蔗遗传改良重点实验室农业部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007

出　　处：《热带作物学报》2013年第11期2222-2226,共5页Chinese Journal of Tropical Crops

基　　金：广西自然科学基金项目(No.2010GXNSFD013034、2011GXNSFF018002、2012GXNSFBA053036);现代农业产业技术体系(No.CARS-20-1-3);广西农科院基本科研业务专项项目(桂农科No.2011YT01、2011YT04、2013YT06)

摘　　要：黑穗病是世界各植蔗区最普遍、最主要的一种气传真菌性病害。为筛选与甘蔗抗黑穗病基因连锁的分子标记,利用高抗和感病亲本杂交的后代群体为材料,分别应用浸渍法和针刺法进行人工接种,通过一年新植一年宿根的抗病性鉴定,采用混合分组分析法(Bulked Segregant Analysis,BSA)构建了抗感池,并结合SSR(Simple Sequence Repeat)分子标记技术筛选出一个与甘蔗抗黑穗病性基因连锁的SSR标记,该标记在甘蔗抗黑穗病育种的辅助选择中具有应用潜力。Sugarcane smut caused by Sporisorium scitaminea Syd.is one of the most severe disease in the world.To screen molecular markers linked to smut resistance gene in sugarcane,hybrids derived from a cross of GT21× ROC25 were taken as the materials and smut resistance was identified by both methods of dipping and puncturing inoculation respectively.With the result of resistance experiment for one-year planting and one-year ratoon cane,a marker linked to resistance gene for sugarcane smut was screened out through bulked segregant analysis （BSA） along with SSR molecular marker.The marker suggested a potential useful role for marker-assisted selection in sugarcane breeding programs.

关 键 词：甘蔗黑穗病 混合分组分析法 SSR分子标记 抗病育种 

分 类 号：S566.1[农业科学—作物学]