抑制性ODN对实验小鼠溃疡性结肠炎的抑制作用研究  

The Effect of Inhibitory ODN on the Mice with ulcerative colitis

作　　者：周晓晶[1] 孙鹏[2] 刘伯岩[1] 杨文颖[2] 孙聪[1] ZHOU Xiao-jing;SUN Peng;LIU Bo-yan(Changchun University of Traditional Chinese Medicine,Changchun 130117,China;Department of Digestive System,Jilin Province People’S Hospital,Changchun 130021,China)

机构地区：[1]长春中医药大学基础医学院生物化学与分子生物学教研室,吉林长春130117 [2]吉林省人民医院消化内科,吉林长春130021

出　　处：《中国实验诊断学》2013年第11期1964-1967,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金面上项目(201015221)

摘　　要：目的研究抑制性ODN对小鼠溃疡性结肠炎的抑制作用。方法首先利用我们已发表的研究[1]中的方法建立小鼠溃疡性结肠炎模型。建模成功后将结肠炎小鼠随机分为三组,分别给予腹腔内注射PBS、A151及A151对照,记作PBS+UC组、A151+UC组和A151对照+UC组,同时设一组健康小鼠对照并给予腹腔注射PBS,记作PBS+健康对照组。每组分别连续注射7天,末次注射后每天观察各组小鼠的状态、大便性状及便隐血情况,并作DAI评分;于末次注射后第7天处死小鼠,分离结肠组织进行组织学评分。结果经A151免疫的溃疡性结肠炎小鼠体重明显增加,而且状态逐渐好转,DAI及结肠组织学评分明显降低,且在末次免疫后7天降低到正常水平。结论说明A151对小鼠溃疡性结肠炎具有抑制作用,展现出治疗溃疡性结肠炎的潜力。Objective To study the Effect of inhibitory ODN on mice with ulcerative colitis.Methods Ulcerative colitis(UC)was induced by DSS in mice.The mice with UC were randomly divided into three groups.Mice with UC were injected with follows by i.p:PBS,A151,A151 control respectively.Disease active index(DAI)was observed every day.At 7 days after last injection the mice were sacrificed and their colons were taken out to undergo histological exami nation.Results The weight gain of mice with UC was induced by A151.Moreover,the DAI score and histological score in the group of A151+UC were significantly lower than those in other UC groups(P〈0.05).Conclusion Inhibitory ODN might have the potency to therapy UC.

关 键 词：溃疡性结肠炎 抑制性ODN 葡聚糖硫酸钠 

分 类 号：R516.1[医药卫生—内科学]