富铬枯草芽胞杆菌培养条件的探究  

Cultural conditions of chromium enriched Bacillus subtilis

作　　者：杨家军[1] 许月英[1] 章薇[1] 

机构地区：[1]安徽省农业科学院畜牧兽医研究所,安徽合肥230031

出　　处：《粮食与饲料工业》2013年第11期47-50,共4页Cereal & Feed Industry

基　　金：安徽省农业科学院院长青年创新基金项目(编号:13B0427);安徽省农业科学院博士启动项目

摘　　要：通过单因子试验和正交试验对富铬枯草芽胞杆菌的培养条件:培养温度、培养时间、无机铬水平、接种量和初始pH值进行了优化,研究了各因素对枯草芽胞杆菌数量和有机铬转化率的影响。结果表明:在初始pH值7.0、转速200r/min、接种量3%、培养温度37℃、培养时间36h和无机铬水平32.5μg/g条件下,枯草芽胞杆菌数量的对数值为8.84,枯草芽胞杆菌的生物量为1.018g/L,有机铬水平可达1 246.85μg/g。The cultural conditions including temperature, time, inorganic-chromium level, inoculation and initial pH of chro- mium enriched Bacillus subtilis were optimized through single and orthogonal test, and the effects of all the factors on Bacillus subtilis amount and organic chromium conversion rate were studied. The optimum conditions were as that： the initial pH of 6.5,chromium amount of 32.5 ug/g,inoculation of 3%, roll speed of 200 r/min, at 37℃ for 36 h. Under the conditions,the value of Bacillus subtilis number reached 8.84, the biomass was 1. 018 g/L, organic chromium levels up to 1 246.85 ug/g.

关 键 词：铬 枯草芽胞杆菌 饲料 接种量 培养温度 培养时间 小麦粉培养基 

分 类 号：S476[农业科学—农业昆虫与害虫防治]