儿童下呼吸道人类博卡病毒感染的病毒载量和疾病严重程度相关性研究  

The correlation study of HBoV viral load and disease severity in children with lower respiratory tract infections

作　　者：赵百慧[1] 王春[2] 滕峥[1] 沈佳仁[1] 高烨[1] 俞雪莲[1] 张泓[2] 张曦[1] 

机构地区：[1]上海市疾病预防控制中心微生物实验室,上海200335 [2]上海市交通大学医学院附属儿童医院检验科,上海200040

出　　处：《检验医学》2013年第11期1016-1020,共5页Laboratory Medicine

基　　金：上海市卫生局青年科研项目(20124y184);上海市卫生微生物公共卫生重点学科(12GWZX0801)

摘　　要：目的研究下呼吸道感染患儿人类博卡病毒(HBoV)载量与疾病严重程度的相关性。方法对2009年1月至2012年9月间554例因下呼吸道感染住院的5岁以下患儿及195名健康对照组儿童下呼吸道吸出物(NPAs)、鼻咽拭子采用荧光聚合酶链反应(PCR)检测HBoV种类,并同时测定阳性标本感染HBoV的载量,利用商品化试剂盒检测其他种类的呼吸道病毒,结合患儿的临床症状和生化指标进行综合分析。结果实验组与健康对照组HBoV阳性率有明显差异,实验组病毒载量(5.10×106拷贝/mL)明显高于对照组(2.70×103拷贝/mL,P<0.05)。实验组中混合感染组疾病严重程度为一般和中等的患儿HBoV载量(分别为2.70×105、3.85×106拷贝/mL)明显低于严重患儿(8.70×106拷贝/mL,P<0.05)。在1例新生儿重症肺炎患儿NPAs、血液和粪便中均检测到HBoV,并排除了其他常见呼吸道病毒和细菌感染。结论 HBoV是呼吸道疾病致病病原,混合感染中HBoV载量与疾病严重程度呈正相关,HBoV在某些患儿中可以导致重症肺炎。Objective To study the correlation of hmnan bocavirus （HBoV） viral load and disease severity in children with lower respiratory tract infections. Methods Nasopharyngeal aspirates （ NPAs ） from 554 in-hospital children with lower respiratory tract infections under 5-year-old and swabs from 195 healthy children were collected from January 2009 to September 2012. The types of HBoV and HBoV viral load were determined by fluorescence polymerase chain reaction （PCR）. The other respiratory viruses were determined by commercial kits. The results were analyzed with clinical symptoms and biochemistry parameters. Results The positive rates of HBoV between in-hospital children and healthy children had obvious difference. The HBoV viral loads of in-hospital children （5. 10 xl0^6 copies/mL） were significantly higher than those of healthy children （2.70 x 103 eopies/mL, P 〈 O. 05 ）. Among co-infection in-hospital children, the HBoV viral loads of mild and moderate groups （2.70 x 10^5 and 3.85 x 10^6 eopies/mL） were lower than that in severe group （8.70 x 10^6 eopies/mL, P 〈0.05 ）. There was 1 ease of newborn infant with severe pneumonia, and HBoV were found in NPAs, blood and stool samples without other common respiratory trraet virus and bacterium infections. Conclusions HBoV is a pathogen for respiratory tract disease in children. HBoV viral load is correlated positively with disease severity in co-infection patients. HBoV can cause severe pneumonia in some children.

关 键 词：人类博卡病毒 病毒载量 重症肺炎 儿童 

分 类 号：R373.1[医药卫生—病原生物学]