一种基于S-层蛋白基因检测炭疽芽胞杆菌PCR方法的研究与建立  

Establishment of PCR based on S-layer protein gene for detecting Bacillus anthracis

作　　者：顾贵波[1] 赵殿国 赵凤菊[1] 郑洪玲[1] 吴运谱[1] 冯书章[3] 

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110164 [2]辽宁省鞍山市动物疫病预防控制中心,辽宁鞍山114014 [3]军事医学科学院军事兽医研究所,吉林长春130162

出　　处：《现代畜牧兽医》2013年第11期69-72,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：鞍山市科技计划项目(2012039)

摘　　要：在国内外的炭疽芽胞杆菌检测方法中,针对炭疽芽胞杆菌基因的检测方法,其特异性以及临床的实用性方面难以令人满意,特别是针对炭疽疫情在临床诊断及鉴别诊断方面还有欠缺。本文针对炭疽芽胞杆菌的S层蛋白建立一种PCR检测方法,并初步应用于我省的疫情临床诊断。结果表明,所建立的PCR方法,能检测到100 pg/μL级,对其他芽胞杆菌无交叉反应,检测特异性和敏感性强。该方法适合于现场疫情的快速诊断。Among methods both at home and abroad for detecting Bacillus anthracis the methods targeting genes of Bacillus anthracis are far from satisfactory in speci ficity and clinical practicality, which are short in clinical diagnosis and differen tiate diagnostic for Bacillus anthracis outbreak, especially. The purpose of this pa per is to establish a PCR method for detecting Bacillus anthracis based on S layec protein gene, and then preliminary applied it for rapid diagnosis of Bacillus anthra cis outbreak in Liaoning province. The results indicated that the sensitivity of the PCR method based S-layer protein of Bacillus anthracis was determined as 100 pg/μL, without cross reactions to other kinds of Bacillus, with high sensitivity and specificity. The established PCR provided a sensitive method for the rapid diagnosis of Bacillus anthracis outbreak.

关 键 词：炭疽芽胞杆菌 S层蛋白 PCR 

分 类 号：S852[农业科学—基础兽医学]