晚期糖基化终末产物对糖尿病结膜松弛症的影响  

Effect of advanced glycation end produts in diabetic conjunctivochalasis

作　　者：江兵[1] 徐智勇[2] 王安素[1] 刘苏[2] 

机构地区：[1]遵义医学院附属医院眼科,贵州遵义563099 [2]重庆医科大学附属第二医院眼科,重庆400010

出　　处：《遵义医学院学报》2013年第5期449-452,共4页Journal of Zunyi Medical University

基　　金：重庆市教委科学技术研究基金项目(NO:KJ060319)

摘　　要：目的测定晚期糖基化终末产物(AGEs)在糖尿病结膜松弛患者结膜组织及血清中的含量,探讨其在糖尿病结膜松弛症发生过程中的作用机制。方法共收集结膜标本36份(糖尿病结膜松弛症6例9眼、单纯结膜松弛症5例9眼、糖尿病不伴结膜松弛症者9例9眼和正常人9例9眼),血清标本29份(每人静脉采血),采用竞争性酶联免疫吸附试验法(ELISA)定量测定其AGEs含量。结果在糖尿病结膜松弛组、糖尿病组两组血清AGEs含量(ng/ml)分别为17.5525±4.1593和15.7092±3.1896,明显高于结膜松弛组和正常结膜组的12.0809±3.2551和9.0161±2.4255,差异有统计学意义(P<0.05);在糖尿病结膜松弛组与糖尿病组、结膜松弛组与正常结膜组病组,血清AGEs含量差异无统计学意义(P=0.089)。结膜组织中AGEs含量在糖尿病结膜松弛组(0.4240±0.0903)、糖尿病组(0.3436±0.0780)、结膜松弛组(0.2682±0.0410)、正常结膜组(0.1895±0.0176)依次降低,各个组间差异均有统计学意义(P<0.05)。结论血清及结膜中AGEs水平升高与糖尿病结膜松弛症密切相关,高糖环境和AGEs蓄积可能在糖尿病结膜松弛的发生和发展中起到重要作用。Objective This study was to investigate the level and role of AGEs in diabetic conjunctiv- ochalasis. Methods The levels of AGEs were assessed by competitive enzyme - linked immunosorbent assay （ELISA） in 36 conjunctive tissue samples and 29 serum samples which were collected from the patients with diabetic conjunctivochalasis （9 eyes of 6 cases）, only conjunctivochalasis （9 eyes of 5 ca- ses）, diabetes without conjunctivochalasis （9 eyes of 9 cases） and normal subjects received extraeapsu- lar extraction （9 eyes of 9 cases）. Results Mean levels of serum AGEs （ng/mL） in diabetic eonjunctiv- ochalasis and diabetes were 17. 5525 ±4. 1593 and 15. 7092 ± 3. 1896, showing a significantly higher level than those of only conjunctivochalasis group and normal conjunctiva group （ 12. 0809 ± 3. 2551, 9. 0161 ±2. 4255 ） （P 〈 0.05 ）. There was no statistically significant difference in the level of serum AGEs in diabetic eonjunctivochalasis group and diabetes group （ 17. 5525 ± 4. 1593 vs 15. 7092 ± 3. 1896）, conjunctivoehalasis group and normal conjunctival group （ 12. 0809 ± 3. 2551 vs 9. 0161 ± 2.4255 ）. The levels of AGEs （ng/mg） in conjunctive tissue decreased gradually in diabetic onjunetiv- ochalasis group（0.4240 ± 0. 0903 ） , in diabetes group （0. 3436 ± 0. 0780 ）, conjunctivochalasis group （0.2682 ± 0.0410） and normal eonjunctival group （0.1895 ± 0.0176 ）. There was statistically signifi- cant difference in the level of AGEs in conjunctive tissue between any two group （P 〈0.05 ）. Conclu- sion The higher levels of AGEs in serum and conjunctival tissue were closely correlated with diabetic eonjunctivochalasis. High glucose and AGEs accumulation may play a critical role during the occurrence and development of diabetic conjunctivochalasis.

关 键 词：结膜松弛症 糖尿病 晚期糖基化终末产物 

分 类 号：R544.1[医药卫生—心血管疾病]