人巨细胞病毒糖蛋白B基因分型与婴幼儿感染致病的相关性研究  

Association of Human Cytomegalovirus Genotyping with Infant Infection

作　　者：易思思[1] 叶志纯[1] 孙开胜[1] 李梨平[1] 

机构地区：[1]湖南省儿童医院儿科研究所,湖南长沙410007

出　　处：《实用预防医学》2013年第11期1301-1303,共3页Practical Preventive Medicine

基　　金：湖南省科技厅基金项目(2009FJ3119)

摘　　要：目的探讨不同人巨细胞病毒糖蛋白B基因分型与婴幼儿感染致病间的关系。方法临床采集200例婴幼儿感染HCMV血液标本,采用巢式PCR检测130例婴儿肝病综合征患者、33例血小板减少症患者和37例神经性耳聋患者标本中HCMV的gB基因,并通过限制性片段长度多态性分析(RFLP)对gB基因进一步分型。分析HCMV及其不同gB基因型与上述婴幼儿感染致病的关系。结果婴儿肝病综合征患者中gB基因型主要以gB1型为主,占56.9%;血小板减少症患者中gB基因型分布较均衡,各gB分型无特异性;而在神经性耳聋患者中,gB基因型主要为gB4型,占40.5%,其次为gB1型占24.3%。不同gB基因型HCMV感染患者在婴儿肝病综合征和神经性耳聋中的分布差异有统计学意义(P<0.05)。结论婴儿肝病综合征和神经性耳聋与HCMV的gB分型密切相关,而血小板减少症无直接关系。Objective To explore the relationship between different human cytomegalovirus （HCMV） glycoprotein B （gB） genotyping and infant infection.Methods Blood samples were collected from 200 infants with HCMV infection,including 130 cases with infant liver disease syndrome,33 cases with thrombocytopenia and 37 cases with nervous deafness.HCMV gB gene was detected by nested PCR and was further genotyped by restriction fragment length polymorphism （RFLP）.The associations of different HCMV gB genotypes with infant infection were analyzed.Results For the patients with infant liver disease syndrome,the gB1 genotype was the most frequent,accounting for 56.9％.For the cases with thrombocytopenia,the distribution of the gB genotypes was more balanced while for the nervous deafness patients,the gB4 genotype was the most frequent （40.5％）,followed by 24.3％ of genotype gB1.The distribution of various gB genotypes was statistically different between the patients with infant liver disease syndrome and those with nervous deafness （P ＜ 0.05）.Conclusions Infant liver disease syndrome and nervous deafness are closely related with HCMV gB genotypes,but thrombocytopenia is not.

关 键 词：人巨细胞病毒糖蛋白B gB基因型 聚合酶链反应 RFLP 

分 类 号：R722.1[医药卫生—儿科]