氯化镉对条斑星鲽卵巢细胞的毒性作用及其机理研究  

The cytotoxic effects of cadmium on barfin flounder ovary cells and its mechanism study

作　　者：徐晓辉[1] 樊廷俊[1] 景毅[1] 姜国建[1] 杨秀霞[1] 葛源[1] 

机构地区：[1]中国海洋大学海洋生命学院海洋生物系,山东青岛266003

出　　处：《山东大学学报（理学版）》2013年第11期1-6,共6页Journal of Shandong University(Natural Science)

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA02A132)

摘　　要：使用不同浓度的氯化镉(CdCl2)处理体外培养的条斑星鲽卵巢(BFO)细胞系细胞,利用毒理学和细胞生物学方法研究了重金属镉对BFO细胞的细胞毒性及其作用机理。毒性作用研究结果显示,BFO细胞对CdCl2敏感,浓度大于10μmol/L的CdCl2对细胞的生长具有显著的抑制作用,能引起细胞内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GST-Px)活性的持续降低和丙二醛(MDA)含量的持续升高,且具有浓度依赖性;机理研究结果显示,浓度大于40μmol/L的CdCl2可引起BFO细胞出现典型的凋亡细胞形态,BFO细胞在AO/EB荧光双染色中的质膜通透性显著提高,在单细胞凝胶电泳中出现明显的彗尾,且细胞凋亡率、彗尾的长度和亮度随CdCl2浓度的增加而逐渐升高,并具有浓度依赖性。可见,镉对BFO细胞具有显著的毒性作用,其毒性作用是通过诱导细胞凋亡来实现的,为利用BFO细胞系研究镉等重金属的细胞毒性及其作用机理奠定了基础。To investigate the cytotoxicity and its underlying mechanisms of cadmium chloride （ CdC12 ） to barfin floun- der ovary （BFO） cells, in vitro cultured BFO cells were treated with CdC12 at different concentrations and investigated toxicologically and cytologically in this study. Cytotoxicity results showed that the BFO cells were very sensitive to CdC12, and their cell growth were inhibited in a concentration-dependent manner by CdC12 at a concentration above 10 ixmol/L. CdC12 at a concentration above 40 ixmol/L could reduce the activities of superoxide dismutase （SOD） and glutathione peroxidase （ GST-Px ）, and elevate the content of melondialdehyde （MDA） in BFO cells significantly. Mechanism results showed that typical morphological changes similar to those of apoptotic cells were found in BFO cells after treated with CdC1z at a concentration above 40 p^mol/L. Obvious elevation of plasma membrane permeability in AO/EB double fluorescent staining and comet tails in single cell gel electrophoresis were both found in CdC12 treated BFO cells. And the apoptotic ratio of BFO cells, the length and fluorescent intensity of comet tails from BFO cells were increased with the concentration of CdC12. In conclusion, CdC12 has significant cytotoxicity to BFO cells which was achieved by inducing cell apoptosis, which has laid a solid foundation for studies of the cytotoxicity and toxic mecha- nisms of cadmium and the other heavy metals.

关 键 词：氯化镉 条斑星鲽卵巢细胞系 细胞毒性 细胞凋亡 

分 类 号：X503.225[环境科学与工程—环境工程] Q813.11[生物学—生物工程]