四川地区HBsAg阳性孕妇HBV血清免疫模式与PreS1-Ag、HBV DNA相关性分析  

Correlation of HBV immunological modes,PreS1-Ag,and HBV DNA in HBsAg positive pregnant women in Sichuan

作　　者：朱巧英[1] 李宁[1] 陈志蛟 梁蓉[1] 成春兰[1] 

机构地区：[1]四川省妇幼保健院检验科,四川成都610031 [2]成都军区总医院,四川成都610083

出　　处：《国际检验医学杂志》2013年第22期3035-3036,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨四川地区HBsAg阳性孕妇HBV血清免疫模式与PreS1-Ag、HBV DNA之间的相关性。方法分别用时间分辨免疫荧光法、ELISA法对512例HBsAg阳性孕妇的血清进行HBV血清免疫模式、PreS1-Ag检测,同时采用实时荧光定量PCR检测HBV DNA。结果 512例标本中:A组(HBsAg、HBeAg、HBcAb阳性)有178例,其血清中HBV DNA、PreS1-Ag阳性率分别为98.31%、96.62%;B组(HBsAg、HBeAb、HBcAb阳性)有255例,其血清中HBV DNA、PreS1-Ag阳性率分别为41.17%、37.25%;C组(HBsAg、HBcAb阳性)有68例,其血清中HBV DNA、PreS1-Ag阳性率分别为35.29%、29.41%;D组(HBsAg、HBsAb、HBeAg、HBcAb阳性)有8例,其血清中HBV DNA、PreS1-Ag阳性率分别为87.50%、75.0%;E组(HBsAg、HBsAb、HBeAb、HBcAb阳性)3例,其血清中HBV DNA、PreS1-Ag阳性率分别为66.67%、66.67%。结论 HBV DNA、PreS1-Ag符合率较高,联合检测HBV血清免疫模式、HBV DNA、PreS1-Ag,能更正确反映乙型肝炎病毒的复制状态和活跃程度。Objective To study the relativity of Pre S1, HBV-DNA and HBV immunological modes of HBsAg positive parturi ent women in Sichuan. Methods FQ-PCR and EI.ISA were used to detect HBV immunological modes and Pre-S1 of 512 cases of HBsAg positive parturient women. FQ-PCR was used to detect HBV DNA. Results The positive rates of HBV-DNA and Pre-S1 antigen were respectively 98.31% and 96.62% among 178 cases with HBsAg, HBeAg,and HBcAh positive（group A）. The posi- tive rates of HBV-DNA and Pre-S1 antigen were respectively 41. 17% and 37.25% among 255 cases with HBsAg, HBeAb, and HBcAb positive（group B）. The positive rates of HBV-DNA and Pre-S1 antigen were respectively 35.29 % and 29.41 % among 68 cases with HBsAg and HBcAb positive（group C）. The positive rates of HBV-DNA and Pre-S1 antigen were respectively 87.50% and 75.0% among 8 cases with HBsAg, HBsAb, HBeAg,and HBcAb positive （group D）. The positive rates of HBV-DNA and Pre-S1 antigen were respectively 66.67% and 66. 67% among 3 cases with HBsAg, HbsAb, HBeAb, and HBeAb positive（group E）. Conclusion HBV-DNA has a higher coincidence rate with Pre-S1 antigen. Joint detection of HBV-DNA, PreS1-Ag, and HI3V immunological modes could be able to reflect HBV replication status more accurately.

关 键 词：HBSAG阳性 前S1抗原 HBV DNA 孕妇 

分 类 号：R446.6[医药卫生—诊断学]