鳖甲寡肽I-C-F-6抗酒精诱导小鼠急性肝损伤活性研究  

Bioactive study of Oligo-peptide I-C-F-6 in Carapax Trionycis on acute liver injury induced by alcohol in rats

作　　者：徐士勋[1] 王咪娜[2] 林锦旋[2] 程亚涛[1] 王艳慧[1] 绪扩[1] 张宇忠[2] 雷海民[1] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102 [2]北京中医药大学基础医学院,硕士研究生100102

出　　处：《环球中医药》2013年第10期721-724,共4页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81073017);北京中医药大学科研创新团队项目(2011-CXTD-15)

摘　　要：目的探讨鳖甲寡肽I-C-F-6对60%酒精诱发的小鼠急性肝损伤的保护作用。方法将48只ICR小鼠随机分为正常对照组、模型组、寡肽低剂量组(0.12 mg·kg-1)、寡肽高剂量组(0.24mg·kg-1)、阳性药组(240 mg·kg-1)。正常对照组和模型组均皮下注射生理盐水(0.12 ml·kg-1);寡肽低剂量组和寡肽高剂量组分别皮下注射寡肽的生理盐水溶液(0.12 ml·kg-1);阳性药组灌胃还原型谷胱甘肽生理盐水溶液(0.12 ml·kg-1),每天1次,连续30天。30天后,除正常对照组外,其余组均灌胃60%乙醇3次(10小时/次)(0.12 ml·kg-1),末次给酒精12小时后处死小鼠采集血液样品和肝组织样品,测定血清谷丙转氨酶(ALT)和谷草转氨酶(AST)、肝组织超氧化物歧化酶(SOD)活力、丙二醛含量(MDA),并进行病理组织学检查。结果鳖甲寡肽I-C-F-6使灌胃酒精后小鼠血清ALT、AST含量和肝匀浆MDA含量显著降低(P<0.05),肝匀浆SOD活力显著升高(P<0.05),肝脏病理损伤减轻。结论鳖甲寡肽I-C-F-6对小鼠急性酒精性肝损伤具有一定的预防保护作用。Objective To study the protective effect of oligo-peptide I-C-F-6 on the acute liver injury induced by alcohol in rats. Methods 48 healthy ICR rats were randomly divided into five groups： normal control group, model group, oligo-peptide low dose group（0.12 mg·kg^-1） , oligo-peptide high dose group（0.24 mg·kg^-1） , positive group（240 mg·kg^-1）. Rats in oligo-peptide groups were administered oligo-peptide＇ saline solution （0.12 ml·kg^-1 ） once a day for 30 days, while rats in normal group and model group were given saline solution（0.12 ml ·kg^-1 ） ; positive group（240 mg·kg^-1） was given reduced-glutathione（ 240 mg·kg^-1 ） by intragastric administration once a day for 30days too. After 30 days,rats in treatment groups and model group were given 60% alcohol 3 times（0.12 ml·kg^-1 ） ,which the interval is 10 hours, then after 12 hours when the third time finished, they were sacrificed to collect the blood samples and liver tissue samples. At last, the levels of aminotransferase （ALT） , aspartate aminotransferase （AST） and the levels of liver tissue superoxide dismutase （SOD） , malonicdialdehyde（MDA） were determined respectively, changes of pathohisology of rats were observed. Results Serum concentrations of ALT, AST and was significantly higher in acute liver injury model group. Compared with model group, the serum concentrations of ALT, AST and the liver tissue content of MDA was significantly decreased in treatment groups （ P 〈 0.05 ） ; the activity of SOD in the liver tissue in treatment groups were also significantly increased （ P 〈 0.05 ）. Pathological result showed that the acute liver injury in treatment groups was significantly improved. Conclusion Oligo-peptide I-C-F-6 has a significant therapeutic effect on acute liver injury induced by 60% alcohol in rats.

关 键 词：寡肽I-C—F-6 急性酒精性肝损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]