基于全转录组测序技术的转录组学在中医药领域的应用前景分析  

Application prospect analysis in the field of traditional Chinese medicine based on the transcription of RNA-seq technology

作　　者：陈惠[1] 辛丽丽[1] 龚婕宁[1] 

机构地区：[1]南京中医药大学基础医学院温病教研室,硕士研究生210046

出　　处：《环球中医药》2013年第10期759-763,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81373603)

摘　　要：转录组学以其在全基因组转录水平上整体性、系统性的研究特点在中医药研究中发挥了重要作用,近年来兴起的全转录组测序(RNA Sequencing,RNA-Seq)技术使转录组学研究进入了数字化、信息化的时代。本文总结归纳了RNA-seq技术在转录组学中的优势,其整体性、系统性、个体组织差异性、时间独立性等优势改善了以往单基因研究不能切合中医学整体观念的不足;着重探讨了基于RNA-seq技术的转录组学在中医学理论及方药研究等方面的应用现状及前景,对其在中医辨治理论实质研究、方药有效成分的筛选和作用机制研究及临床用药安全评定体系等方面的应用策略进行了分析,并提出了初步的实验方案。For the feature of integrity and systematicness on the level of the whole genome, the transcriptomics play a significant role in research of Chinese medicine, the recently arisen RNA deep sequencing technology（RNA-seq） allows for the age of digital and information. In this paper, we summarize the advantages of RNA-seq in the transcriptomies, its integrity, systematicness, individual tissue differ- ences, time independence and many other edges have solved the previous research problem that single gene research can＇t failed to correspond with the perspective of holism in traditional Chinese medicine （TCM）. This paper discusses the transefiptome study based on the technology of RNA-seq in TCM theory and the formulas and medicine about their present situation in application and prospect. Analysis has been made on the application strategy in the research of syndrome differentiation in TCM, the selection of active ingredients of formulas and medicine and the research of mechanism, clinical drug safety evaluation system, and a preliminary experimental scheme was proposed.

关 键 词：中医药研究 分子生物学 转录组学 全转录组测序技术 

分 类 号：R34[医药卫生—基础医学]