灵发素(LFS)对诱导甘蔗愈伤组织的影响  

Influence of LFS on Inducing Sugarcane Callus

作　　者：陈念平[1] 蓝桃菊[1,2] 龚银花[1] 解云英[3] 许鸿章[3] 梁琼月[1] 周凤珏[1] 何冰[1] 许鸿源[1] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西农业科学院,南宁530007 [3]中国医学科学院医药生物技术研究所,北京100050

出　　处：《种子》2013年第11期1-2,7,共3页Seed

基　　金：国家“重大新药创制”科技重大专项(编号:2012 ZX09301002-001-018);国家“863”专项经费(2006 AA 10 A213)资助

摘　　要：目的:建立高效率诱导甘蔗优质愈伤组织的技术。方法:以甘蔗幼叶作外植体,MS为基本培养基,分别添加不同浓度的新型诱导剂LFS与传统的诱导剂2,4-D进行对比研究。结果:LFS 0.05～1.00 mg/L均能高效优质地诱导愈伤组织,其适宜浓度为0.20 mg/L,培养20 d,无需更换新鲜培养基,出愈率达98%,褐化、板结轻微,转绿率达16.8%,效果显著优于2,4-D。结论:LFS可以比2,4-D更高效优质地诱导甘蔗愈伤组织,并显著简化生产程序。Objective：To establish the technology for inducing efficiently the sugarcane callus with high quality. Methods： Take young leaf cuts of sugarcane as explants, and MS as basic medium added respectively different concentrations of new inductive agent LFS, and with the traditional inductive agent 2,4-D as ek for contrast research. Results： MS ＋ LFS 0.05 - 1.00 mg/L could all induce callus with high efficient and quality. The suitable concentration of LFS was 0.20 mg,/L, cultivated 20 d, callus induction rate reached 98%, browning and hardening slightly,greenish rate reached 16.8%. Such callus was very suitable for organ differentiation in further. Conclusion：LFS is better than 2,4-D to induce efficiently sugarcane callus with high quality and simolifin significantly the oroductive orocess.

关 键 词：甘蔗 灵发素 愈伤组织 

分 类 号：S482.8[农业科学—农药学]