多溴联苯醚47对日本青鳉神经行为发育的影响  

Neurobehavioral derangement in medaka fish receiving polybrominated diphenyl ethers( PBDE-47) during the early-stage development

作　　者：吴源[1] 郑田利[1] 王静雅[1] 张雅莉[1] 陈婷婷[1] 

机构地区：[1]安徽医科大学公共卫生学院,合肥230601

出　　处：《卫生研究》2013年第6期932-936,共5页Journal of Hygiene Research

基　　金：安徽省高校优秀青年人才基金(No.2009SQRZ040);安徽医科大学博士科研启动基金(No.XJ201016);国家自然科学基金(No.81101272);国家级大学生创新创业训练计划项目(No.201210366005)

摘　　要：目的评价多溴联苯醚47（PBDE-47）对小型鱼日本青鲋（medaka）神经行为发育的影响。方法日本青鲋孵化后第1天分为PBDE--47暴露组（50、150、450及1350ng／L）、空白对照组和溶剂对照组，随机抽取各组幼鱼，在孵化后第5、10及60天分别测定惊觉反应、眼动反应（OKR）、视动反应（OMR）、背光反应（DLR）和肥满度。结果除最高剂量组幼鱼死亡率增加，其余各处理组一般发育指标无明显差异；各组肥满度在0．916～1．042。幼鱼惊觉反应与避险反应阳性率呈剂量依赖性下降。与对照组比较，较高浓度组OMR和DLR反应时间明显延长，差异有统计学意义（P〈0．05或P〈0．01），但是各组OKR指标无明显变化。结论PBDE-47抑制发育早期幼鱼的知觉一行为协调能力，其机制与PBDE内分泌干扰作用有关。Objective To investigate the neurobehavioral toxicity of polybrominated diphenyl ethers（ PBDE-47 ）to larval medaka during the early-stage development. Methods Early-life stage fish were treated with PBDE-47, control, and solvent control. Animals were randomly collected on 5, 10 and 60 days after exposure, and some toxicological endpoints such as startle response, optokinetic response （OKR）, optomotor response （OMR） and dorsal light response （DLR） were measured. Results There were no any significant difference in body weight gain or body length in the PBDE-47 treated medaka except for the highest concentration. Both startle response and escape response were inhibited in dose-related manners. Furthermore, delayed OMR and DLR were induced in the higher PBDE-47 groups （ P 〈0.05 or P 〈0.01 ）. However, OKR was similar between the each group. Conclusion Harmony of the sense-behavior in the medaka larvae could be impaired by PBDEs, and further study should focus on the relationship between the toxicity and hormone disruption caused by PBDE-47.

关 键 词：多溴联苯醚47 日本青鳉 神经发育毒性 

分 类 号：R994.6[医药卫生—毒理学] Q593.2[医药卫生—药学]