氟对原代培养大鼠睾丸支持细胞氧化应激和凋亡的影响  

Effects of fluoride on oxidative stress and apoptosis in primary culture of rat sertoli cells

作　　者：杨阳[1] 黄辉[1] 袁伟[1] 冯得敏 王志谨[1] 程学敏[1] 巴月[1] 崔留欣[1] 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,郑州450001

出　　处：《卫生研究》2013年第6期1004-1007,共4页Journal of Hygiene Research

基　　金：河南省教育厅科学技术研究重点项目(No.13A330735)

摘　　要：目的研究氟化钠对大鼠睾丸支持细胞氧化应激和凋亡水平的影响。方法采用原代细胞培养方法,将大鼠睾丸支持细胞暴露于0、6、12和24μg/ml的氟化钠中,24 h后检测支持细胞内活性氧(ROS)、丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性,线粒体膜电位及凋亡率水平。结果氟可降低睾丸支持细胞活力(P<0.01);与对照组相比,各氟化钠处理组的ROS水平均显著上升(P<0.01),12和24μg/ml氟化钠组的MDA含量显著上升(P<0.01)。同时,各氟化钠处理组的SOD活性均显著低于对照组(P<0.05或P<0.01)。随着氟化钠剂量的增高,各氟化钠处理组的线粒体膜电位均显著下降,细胞早期凋亡率均显著升高(P<0.01)。结论氟可致睾丸支持细胞氧化应激水平及凋亡率升高。Objective To study effects of fluoride on oxidative stress and apoptosis in rat sertoli cells. Methods Intracellular reactive oxygen species （ROS） , malondialdehyde （MDA） content, superoxide dismutase （SOD） activity, mitochondrial membrane potential and percentage of apoptosis were measured after the rat sertoli cells were incubated with 0,6,12 and 24 μg/ml sodium fluorides or 24 hours in vitro. Results Fluoride decreased the cell activity significantly （P 〈 0.01 ）. The results suggested that exposure to fluoride significantly increased the level of ROS and MDA content（ P 〈 0.01 ） , fluoride also decreased SOD activity significantly （P 〈 0.05 or P 〈 0.01 ）. In addition, with the NaF dose increased, there is a significantly decreasing in mitochondrial membrane potential and a significantly increasing in early apoptosis rate （P 〈 0.01 ）. Conclusion Fluoride can induce excessive oxidative stress and increased apoptosis rate in rat sertoli cells.

关 键 词：氟化钠 睾丸支持细胞 氧化应激 凋亡 

分 类 号：R994.6[医药卫生—毒理学] Q343[医药卫生—药学]