雷公藤内酯醇诱导SKM-1细胞凋亡机制研究  

Study on the mechanism of triptolide inducing the apoptosis of SKM-1 cells

作　　者：华海应[1] 高华强[1] 孙爱宁[2] 朱文艳[1] 姜东林[1] 岑建农[2] 吴丽丽[2] 

机构地区：[1]南通大学第三附属医院血液科,江苏省无锡市214041 [2]苏州大学第一附属医院血液科,215006

出　　处：《实用医学杂志》2013年第22期3630-3632,共3页The Journal of Practical Medicine

基　　金：江苏省中医药局基金项目(编号:LZ09103);无锡市中医药局基金项目(编号:ZZD0802)

摘　　要：目的：探讨雷公藤内酯醇（triptolide）诱导骨髓增生异常综合征（MDS）细胞系SKM-1细胞凋亡及机制。方法：Triptolide与SKM-1细胞共育，观察细胞形态，流式细胞术检测细胞凋亡，RT—PCR检测Bcl-2、Bax与caspase-3基因表达。结果：40—160ng／mLtriptolide明显促进SKM．1细胞凋亡，随triptolide浓度增加及时间延长，凋亡发生率增加（P〈0．01），Bcl-2表达下降（P〈0．01），Bax、caspase-3表达上升（P〈0．01）。结论：40～160n／mL-triotolide对SKM-1细胞有促凋亡作用．可能通过下调Bcl-2、上调Bax、caspase-3基因表达起作用．Objective To investigate the mechanism of triptolide inducing the apoptosis of myelodysplastic syndrome （MDS） cell line SKM-1. Methods SKM-1 cells were incubated with triptolide, and then the cellular morphology was observed, flow cytometry analysis was used to detect the apoptosis, RT-PCR was used to detect the expressions of Bcl-2, Bax and caspase-3. Results 40-160 ng/mL of triptolide could obviously promote the apoptosis of SKM-1 ceils. With the increase of the acting time and concentration of triptolide, the apoptosis rate was increased （P 〈 0.01）, the expression of Bcl-2 was decreased （P 〈 0.01）, and the expressions of Bax and caspase-3 were increased （P 〈 0.01 ）. Conclusion 40-160 ng/mL of triptolide may promote the apoptosis of SKM-1 cells through down-regulating the expression of Bcl-2 and up-regulating the expressions of Bax and caspase-3.

关 键 词：骨髓增生异常综合征 雷公藤内酯醇 细胞凋亡 Bcl-2 BAX CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]