转化生长因子-β1和骨桥蛋白在肝纤维化大鼠肝组织的表达及荔枝核总黄酮的干预  

The expression of transforming growth factor-β1 and osteopontin in hepatic tissue of rats with hepatic fibrosis and the intervention of total flavone of Litchi Chinensis Sonn

作　　者：黄大健[1] 赵永忠[1] 卢青[1] 周英琼[2] 肖绪华[1] 成秋宸[1] 董勇[1] 何志国[1] 

机构地区：[1]桂林医学院附属医院消化内科,541001 [2]桂林医学院附属医院消病理科,541001

出　　处：《实用医学杂志》2013年第22期3646-3649,共4页The Journal of Practical Medicine

基　　金：广西自然科学基金项目(编号:2012GXNSFAA053 105);广西教育厅科研资助项目(编号:201012MS172);广西卫生厅自筹经费科研课题(编号:Z2011169)

摘　　要：目的:观察荔枝核总黄酮(total flavone of litchi chinensis sonn,TFL)对肝纤维化大鼠肝组织转化生长因子-β1(TGF-β1)和骨桥蛋白(osteopontin,OPN)表达的影响。方法:雄性SD大鼠80只,随机分为假手术组,模型组,TFL大、小剂量组。采用胆总管结扎术制备肝纤维化大鼠模型。术后第4周处死大鼠,放免法检测血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)的水平。HE和Masson染色观察肝组织学变化及纤维化程度;免疫组化观察肝组织TGF-β1和OPN的染色部位及强度。结果:TFL大剂量组血清HA、LN、PCⅢ、CⅣ水平以及肝组织TGF-β1、OPN的表达水平均低于模型组和TFL小剂量组,差异具有统计学意义(P<0.05),假手术组和TFL大剂量组间以及模型组和TFL小剂量组间差异无统计学意义(P>0.05)。结论:大剂量TFL可减轻胆汁淤积性肝纤维化大鼠肝损伤及有效改善肝纤维化程度;抑制TGF-β1、OPN高表达,从而减少细胞外基质(ECM)的分泌可能是其抗肝纤维化的机制之一。Objective To investigate the effects of total flavone of litchi chinensis sonn （TFL） on the expressions of transforming growth factor-β1 （TGF-β1） and osteopontin （OPN） in rats with hepatic fibrosis. Methods Total 80 males SD rats were randomly divided into 4 groups： sham-operated group （SO group）, model group （M group）, high-dose TFL group （HD group）, low-dose TFL group （LD group）. Rat models with hepatic fibrosis were constructed by common bile duct ligation. All rats were sacrificed 4 weeks after the administration, and serum levels of hyaluronie acid （HA）, laminin （LN）, proeolagen type Ⅲ （PC Ⅲ） and Ⅳ collagen （CⅣ） were detected by radioimmunoassay. HE and Masson staining were conducted in hepatic tissues to observe the pathological variations; and the expressions of TGF-β1 and OPN were detected by immunohistochemistry. Results Serum levels of HA, LN, PC Ⅲ and C Ⅳ, and the expressions of TGF-β1 and OPN in hepatic tissues in HD group were significantly lower than those in LD group and M group （P 〈 0.05）. Conclusion High-dose of TFL may effectively ameliorate liver injury and hepatic fibrosis through the mechanisms of inhibiting the high expressions of TGF-β1 and OPN and decreasing the secretion of ECM.

关 键 词：肝硬化 荔枝核 黄酮 转化生长因子-Β 骨桥蛋白 

分 类 号：R285.5[医药卫生—中药学]