含有多个应答逆境元件的合成启动子在水稻中的表达分析  

Expression analysis of a synthetic promoter containing several stress-related elements in transgenic rice plants

作　　者：牟少亮[1,2] 蔡汉阳[1,2] 赖燕[1,2] 马洪丽[1] 何水林[3,2] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]作物遗传改良与综合利用教育部重点实验室,福州350002 [3]福建农林大学作物科学学院,福州350002

出　　处：《植物病理学报》2013年第6期630-635,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30971718;30600391)

摘　　要：存在于启动子中的各种顺式作用元件在基因应答逆境的过程中起重要的作用,对这些元件进行适当组合是构建诱导型合成启动子的重要可行途径之一。本研究利用多个逆境应答元件(S盒、D盒、Gst1盒、TCA盒和LURP1基因启动子的-85^-46区)和CaMV35S核心(-46-+8)序列相连,组成一个人工合成启动子PRBS,并构建了GUS为报告基因的转化载体,通过农杆菌介导的遗传转化方法获得其水稻转基因植株。利用T1代转基因水稻株系,通过检测GUS酶活性,研究了PRBS在不同逆境及外源激素作用下的表达特征。结果发现PRBS组成型表达水平低,可不同程度地应答稻瘟病病原菌侵染,机械损伤和外源激素(ABA、SA和MeJA)处理,表明PRBS可作为诱导型启动子用于水稻抗逆基因工程遗传改良。A variety of cis-acting elements in the promoter region play an important role for gene reponse to stresses. Combination of these elements is a feasible way to construct an inducible promoter. The promoter PRBS was synthesized by combining minimal CaMV35S promoter （-46-＋8） and several stress-related elements （S box, D box, Gstl box, TCA box and 39 bp（-85--46） of the LURP1 promoter, and fused with GUS report gene. The transgenic rice was obtained by using an Agrobacterium-mediated method. Based on the analysis of GUS activity in T1 generation of transgenic rice, the induced expression characteristics of PRBS by different stresses and exogenous hormones were studied. The results showed that PRBS had a very low constitutive expres- sion. It responded differently to incubation of the rice blast fungus, wounding and treatments with exogenous hormones （ABA,SA and MeJA）. It suggested that PRBS could be used to gene engineering for stress resistance in rice as an inducible promoter.

关 键 词：诱导型启动子 生物胁迫 GUS 转基因水稻 

分 类 号：S511[农业科学—作物学]