普城沙雷菌A21-4根际定殖能力及其对辣椒生长和诱导抗病性的影响  

Growth promoting and root colonization ability of Serratia plym uthica A21-4 and its effect on induced resistance in pepper against Phytophthora blight

作　　者：申顺善[1] 常淑娴[1] 朱宏业[1] 王晶晶[1] 张维娜[1] 朴凤植[2] 

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学园艺学院,郑州450002

出　　处：《植物病理学报》2013年第6期636-642,共7页Acta Phytopathologica Sinica

基　　金：河南省重点科技攻关项目(092102110096;122102110042);河南省重大公益性科研项目(081100911300)

摘　　要：本文探讨了辣椒疫病生防菌普城沙雷菌A21-4在辣椒根际的定殖能力及其对辣椒的促生长和诱导抗病性影响。以108cfu·mL-1A21-4菌液处理辣椒苗,A21-4能够有效地定殖在辣椒根际土壤和辣椒根部,移栽第30 d在根际土壤和辣椒根部均保持106cfu·g-1以上定殖密度。辣椒根际土壤中A21-4的定殖密度和病原菌存在与否无显著差异,而辣椒根部A21-4的定殖密度在病原菌存在时显著高于没有病原菌的;A21-4处理有效促进了辣椒地上部和根部的各项生育指标,同时,显著提高了辣椒叶绿素含量和根系活力。A21-4处理的辣椒苗叶绿素含量和根系活力比对照各提高86.1%和481.8%;经A21-4处理后,辣椒根部和叶部的SOD、POD和PAL活性明显提高,辣椒根部的SOD、POD和PAL活性峰值分别比对照提高44.7%、64.2%和77.0%,辣椒叶部SOD、POD和PAL活性峰值分别比对照提高27.9%、134.9%和87.0%;此外,A21-4浸根处理还能够提高辣椒叶部对辣椒疫霉菌的抗性。The colonization ability of Serratia plymuthica A21-4 in pepper rhizosphere and its impact on pepper growth and the induced resistance of pepper to Phytophthora capsici were studied. After 30 days of inoculation of pepper seedlings with 108cfu · mL^-1 bacterial suspension of A21-4, it was found that there was a relative high population density of A2I-4 at I06 cfu · g-1 in rhizosphere. In rhizosphere soil, co-inoculation with pathogen didn＇t affect the colonization density of A21-4; but in pepper roots, colonization density of A21-4 was signifi- cantly stimulated when pathogen existed. Inoculation with A21-4 significantly enhanced the growth index of pep- per, including flesh and dry weight, chlorophyll content and root activity. The chlorophyll content in A21-4 trea- ted pepper plants was increased by 86.1% and root activity was increased by 481.8% ; After inoculation of A21- 4, SOD, POD and PAL activities were all significantly increased up to 44.7%, 64.2% and 77.0% in the pepper roots, and 27.9%, 134.9% and 87.0% in pepper leaves respectively. Meanwhile soaking pepper root with A21-4 suspension induced its resistance to P. capsici.

关 键 词：辣椒疫病 普城沙雷菌A21—4 根际定殖 促植物生长 诱导抗性 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]