三种玉米小斑病菌毒素活性检测方法比较研究  

Comparative Study on the Detection Effects of Three Toxin Activity Detection Methods for Helminthosporium maydis Race C

作　　者：马春红[1] 李秀丽[1,2] 及增发[3] 戴志刚 贾银锁[1] 王立安[2] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北省植物转基因中心,河北石家庄050051 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北省农林科学院,河北石家庄050031 [4]湖北省土壤肥料工作站,湖北武汉430064

出　　处：《云南农业大学学报（自然科学版）》2013年第6期775-779,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：河北省自然科学基金项目(C2011301010);河北省高层次人才“三三三人才工程”项目(A2010003028);河北省科技计划项目(13396401D);农业部“948”项目[2011-G1(3)-07];科技部国际合作项目(2006DFB02480)

摘　　要：利用3种不同的毒素活性检测方法 (离体叶片针刺法、根冠细胞染色法和游离原生质体染色法)检测玉米小斑病菌C小种(HMC)的毒素生物活性,比较其检测效果,用以探讨HMC毒素致病机理。结果表明:离体叶片针刺法虽能简便地测出毒素的活性,但不能做定量分析,仅从宏观上检测出病斑的形成。根冠细胞染色法,不仅定性还可定量分析毒素的活性。游离原生质体染色法,对HMC毒素活性的测定,虽同样准确可靠,但其费用较高。相较而言,根冠细胞染色法对玉米小斑病菌的抗性鉴定是一种简便、快捷、实用的检测方法,且能快速筛选出抗病体,为加速抗病育种提供资源基础。The toxin activity of Helminthosporium maydis race C （HMC） was detected by 3 different methods,and their detection effects were compared.The results showed that isolated leaf acupuncture inoculation could be relatively simple to detect the activity of toxins and lesion formation,but was not carried out for qualitative analysis.The method of root cap cells staining could be used for qualitative as well as qualitative analysis.Although free protoplasts staining method for the determination of HMC toxin activity was accurate and reliable,it cost a lot.In contrast,the method of root cap cell staining is a simple,quick,accurate,economical and applicable method for the determination of disease resistance and can quickly screen the resistant individuals,which provide a material basis for accelerating breeding.

关 键 词：玉米小斑病菌C小种 毒素 活性 检测 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]