侵染美国薄荷的小西葫芦黄花叶病毒的检测与鉴定  

Detection and Identification of Zucchini yellow mosaic virus infecting Monarda didyma L.

作　　者：赵明富[1] 彭晟[1] 杨莹[2] 孙媛[1] 陈微[2] 陈兴[2] 

机构地区：[1]云南农业大学,农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201 [2]红云红河烟草(集团)有限责任公司,云南昆明650202

出　　处：《云南农业大学学报（自然科学版）》2013年第6期898-901,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南中烟工业有限责任公司科技项目(2012JC07)

摘　　要：为明确侵染美国薄荷,引起花叶、叶片扭曲畸形等症状的病毒病原,对采集于云南石林烟庄的美国薄荷采用电子显微镜观察和RT-PCR技术检测。在电子显微镜下观察到大小约760 nm线状病毒粒子。提取总RNA,利用马铃薯Y病毒科特异性简并引物(Sprimer/M4)进行RT-PCR扩增,获得约1 700 bp片段。经测序和序列分析,结果表明:该片段含有1 680个核苷酸,包含部分NIb基因(621 bp)、完整CP基因(837 bp)和3'-UTR区(210 bp)。序列同源性分析的结果显示与小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)分离物(AF127929)的CP氨基酸序列同源性高达98%,与ZYMV分离物(L31350)CP氨基酸序列同源性高达93.5%,表明侵染美国薄荷,引起花叶、叶片扭曲畸形的病毒病原为小西葫芦黄花叶病毒(ZYMV)。In order to ascertain the viral pathogen of infecting Monarda didyma L.which showing the symptom of mosaic,leaf curling,and deformation,the diseased samples from Tobacco finca of Stone forest in Yunnan province were collected and detected with TEM and RT-PCR.Filamentous particles with the length of 760 nm were detected under TEM from diseased M.didyma L.samples.Then Zucchini yellow mosaic virus （ZYMV） was detected in the diseased samples from M.didyma L.by RT-PCR with genera-specific degenerate primers of Potyviridae （Sprimer/M4）,and the 3 end of the genome（1 680 bp） of the virus was cloned and sequenced.Sequence analysis indicated that this fragment contained partial Nib gene （621 bp）,coat protein gene （837 bp） and 3＇-UTR （210 bp）,the CP amino acid sequence of ZYMV BH isolate described in this work shared high identity of 98% with ZYMV isolate （AF127929） and 93.5% with ZYMV isolate （L31350）,respectively.The results show ZYMV is the viral pathogen of inducing he symptom of mosaic,leaf curling,and deformation of M.didyma L.

关 键 词：美国薄荷 小西葫芦黄花叶病毒 RT-PCR 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]