江苏省如东海区条斑紫菜栽培筏架固着Ulva L.属绿藻的分子生物学与形态学分析  

Morphological and molecular analysis of attached Ulva L.green algae from Porphyra rafts from Rudong coasts in Jiangsu Province

作　　者：陈斌斌[1] 马家海[1] 蔡永超[1] 孙彬[1] 高嵩[1] 胡翔[2] 杨建强[2] 

机构地区：[1]上海海洋大学农业部水产种质资源发掘利用重点实验室,上海201306 [2]国家海洋局北海分局,山东青岛266033

出　　处：《海洋环境科学》2013年第3期394-397,共4页Marine Environmental Science

基　　金：国家海洋局公益项目(201205010);国家海洋局重点实验室开放研究基金(MATHAB00000000);科技部国家科技支撑计划(2012BAC07B03)

摘　　要：2011年底紫菜栽培期对江苏如东海区条斑紫菜筏架Ulva L.属固着大型绿藻进行了样品采集与研究,分析了固着绿藻的形态学特征与分子生物学特征。根据形态学分析结果,所采集到的Ulva L.属绿藻样品主要包括Ulva prolifera,U.lin-za,U.compressa,U.flexuosa等4个种类,这与分子生物学的研究结果(主要包括LPP复合体、U.compressa,U.flexuosa等种类)基本一致。但具体的样品通过形态学分析与分子生物学的方法鉴定出来的结果具有差异;其中只有FJ04和FJ09,FJ05和FJ06,FJ07的形态学特征与分子生物学鉴定方法所得结果相同。根据本实验的研究结果,江苏省如东海区条斑紫菜栽培筏架上的大型固着绿藻至少包括U.prolifera,U.linza,U.compressa,U.flexuosa等4个种。部分筏架固着绿藻存在扭曲或皱褶等形态特征,但不具有大量的、复杂的分枝结构,也不具有相当数量的假分枝,与绿潮优势种存在较大的形态学差异。During Porphyra yezoensis cultivation at the end of the year 2011,attached Ulva L.green algae from Porphyra rafts from Rudong coasts in Jiangsu Province were collected and utilized in these researches.Then studies on their morphological characteristics and molecular biology analyses were carried out.According to the results of morphological research,these attached green algae collected mainly consisted of 4 species,including U.prolifera,U.linza,U.compressa,and U.flexuosa.These were in accordance with the results of molecular biological studies that these algae were mainly included in LPP complex,U.compressa and U.flexuosa.But certain samples had different identification results between there morphological and molecular analyses except FJ04,FJ09,FJ05,FJ06 and FJ07.According to this study,the attached Ulva L.green algae from Porphyra rafts from Rudong coasts in Jiangsu Province at least included four species,U.prolifera,U.linza,U.compressa,and U.flexuosa.Some extraordinary morphological characteristics,such as twist or wrinkled axis existed in certain rafts attached Ulva algae.But they neither had massive and complex branches,nor considerable number of fake branches,that＇s the important morphological differentiation with dominant species from green tide.

关 键 词：绿潮 紫菜筏架 来源 形态学 分子生物学 

分 类 号：Q178.53[生物学—水生生物学]