肝移植后长期生存受者外周血淋巴细胞亚群及细胞因子分析  

Analysis on iymphocytes subgroup and cytokines level of the long-term survival patients after OLT

作　　者：贾俊君[1] 王丽[1] 张静[1] 耿磊[1] 周琳[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院肝胆胰外科卫生部多器官联合移植研究重点实验室,杭州310003

出　　处：《中华器官移植杂志》2013年第11期666-670,共5页Chinese Journal of Organ Transplantation

基　　金：国家重点基础研究发展计划（973计划）（2009CB522407）;国家基金创新群体（81121002）

摘　　要：目的探讨肝移植后长期生存受者外周血淋巴细胞亚群及细胞因子的特征。方法用流式细胞术及酶联免疫吸附试验法检测肝移植受者（随访时间超过5年以上者为长期组，随访时间在1年以内者为短期组）及健康人（对照组）外周血T淋巴细胞、B淋巴细胞、自然杀伤细胞（NK细胞）、自然杀伤T淋巴细胞（NKT细胞）的变化，并测定细胞因子白细胞介素9（IL-9）、白细胞介素17（IL-17）及白细胞介素22（IL-22）的水平。结果短期组、长期组、对照组的CD3＋细胞、CD4＋细胞、CD8＋细胞百分率及CD8＋CD28＋细胞／CD8＋细胞值以及IL-9的水平呈逐渐上升趋势。其中，长期组与短期组CD3＋细胞百分率及IL-9水平均低于对照组（P〈0．05），短期组CD8＋细胞百分率低于对照组（P〈0．05），短期组CD8＋CD28＋细胞／CD8＋细胞值低于对照组和长期组（P〈0．05）。长期组NK细胞及B淋巴细胞百分率高于对照组（P〈o．05），长期组IL-22水平高于短期组及对照组（P〈0．05），2组NKT细胞及IL-17的差异无统计学意义（P〉0．05）。结论外周血T淋巴细胞亚群比例及IL-9水平是评价肝移植后受者免疫状态的良好指标，NK细胞、B淋巴细胞及IL-22可能在肝移植术后受者长期生存中发挥着重要角色。Objective To explore the characterization of the long-term survival patients through monitoring the lymphocytes subgroup and cytokines level. Method The lymphocytes subgroups （T, B, and NK） and cytokines （IL-9, -17, -22） of patients and healthy groups were tested by using flow cytometry and ELISA. Results The levels of CD3＋ , CD4＋ , CD8＋ , CD8＋ CD28＋/CD8＋ and IL-9 were gradually increased in the short-term group and long-term group as compared those in healthy group. The percentage of CD3＋ cells and level of IL-9 were significantly lower in short-term group and long-term group than in healthy group （P〈（0. 05）. The percentage of CD8＋ cell was significantly lower in short-term group than in healthy group （P〈0. 05）. The ratio of CD8＋ CD28＋/CD8＋ was significantly lower in short-term group than in long-term group and healthy group （P〈0. 05）. The percentage of B cells and NK cells, and IL-22 were gradually increased in the healthy group, shortterm group and long-term group. The percentage of B cells and NK cells was significantly higher in long-term group than in healthy group （P〈0. 05）. The level of IL-22 was significantly higher in longterm group than in short-term group and healthy group （P〈0. 05）. However, NKT lymphocytes and IL-17 showed no statistically significant difference between long-term group and short-term group. Conclusion The ratio of T lymphocyte subgroups and the level of IL-9 were good biomarkers for evaluating the immune characterization of OLT patients; NK and B lymphocytes, and IL-22 may be associated with the long-term survival in patients after OLT.

关 键 词：肝移植 淋巴细胞 细胞因子 免疫耐受 

分 类 号：R657.3[医药卫生—外科学]