基于光学标测的电除颤实验平台设计与实现  

Design and implementation of optical mapping-based electric defibrillation experimental platform

作　　者：张虹[1] 刘永波[1] 杨昭[2] 杨琳[2] 

机构地区：[1]西安交通大学电气工程学院西安710049 [2]西安交通大学医学院西安710061

出　　处：《仪器仪表学报》2013年第11期2558-2564,共7页Chinese Journal of Scientific Instrument

基　　金：国家自然科学基金(81271661);教育部回国留学基金(第40批);中央高校基本科研业务费专项资金资助项目

摘　　要：根据心脏电除颤实验研究的需要,设计实现了一个基于光学标测原理的实验研究平台。利用波长为519 nm的LED阵列作为激发光源,通过截止波长为600 nm的滤镜、高速CCD摄像头和采集卡提取离体心肌内注射的电压敏感染料所发射的荧光信号,并采用最小均方误差的自适应滤波方法,同步多位点记录到心肌膜电位信号。另外,以ATmega32单片机为主控器件,USB接口,设计了外加直流电场和电刺激脉冲控制模块,使整个实验平台实现了外部干预和信号标测的同步自动控制。本平台不仅为研究电除颤新方法提供了一个良好的实验环境,同时还可用于心律失常的发生机制以及电场对心肌电活动的生物学效应等研究。According to the requirement of cardiac electric defibrillation experiment study,an experiment study plat- form based on optical mapping principle was implemented. In this platform a 519 nm wavelength LED array was used as the exciting light source, the fluorescence signal emitted from the injected voltage-sensitive dye within in vitro myo- cardium was collected through a long-pass filter with cutoff wavelength of 600 nm,a high speed CCD camera and an acquisition card;meanwhile an adaptive filtering method with least mean square error was adopted to eliminate the noise and simultaneously record the multiple myocardium membrane potential signals. In addition, a control module for the delivery of the external electrical field and stimulus pulses was designed using an ATmega32 microprocessor as the main controller and USB interface. The whole platform realizes the synchronous automatic control of the external intervention and signal mapping. The proposed platform not only provides a good experimental environment for the study of the new defibrillation methods, but also can be used for the studies of arrhythmia mechanisms and biological effect of electrical field on cardiac electrical activity.

关 键 词：光标测 电场 脉冲刺激 电除颤 

分 类 号：R318.6[医药卫生—生物医学工程]