Qufeng Tongluo Prescription(祛风通络方)Inhibits Mesangial Cell Proliferation and Promotes Apoptosis through Regulating Cell Cycle Progression  

作　　者：吴喜利 安鹏 叶冰玉 石兴民 孙万森 付荣国 王竹 乔成林 

机构地区：[1]Department of Integrated Traditional Chinese and Western Medicine,Second Affiliated Hospital of Medical School of Xi'an Jiaotong University [2]School of Public Health of Xi'an Jiaotong University [3]Department of Nephrology,Second Affiliated Hospital of Medical School of Xi'an Jiaotong University

出　　处：《Chinese Journal of Integrative Medicine》2013年第12期927-934,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.30801504,No.30901926,No.81100530 and No.81070590)

摘　　要：Objective： To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription （祛风通络方, QFTL） on the regulation of mesangial cells （MCs） proliferation and apoptosis. Methods： The MCs used in this experiment have undergone five passages induced by lipopolysaccharide （LPS）. Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium （MTT） reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction （qRT-PCR）, respectively. Results： The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively （P〈0.05 or P〈0.01）. Moreover, the inhibitory effect is more significant in the QFTL group at 48 h （P〈0.05）. Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase （P〈0.05 or P〈0.01） to cells at S phase （P〈0.01）, implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group （P〈0.05）, while QFTL and Benazepril serum increased the level of MC apoptosis （P〈0.01）. Moreover, the difference between the QFTL group and the Benazepril group was statistically significant （P〈0.01）. Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 （CDK2） and p21 were significantly increased （P〈0.05 or P〈0.01）, p27 was decreased but with no statistical significance （P〉0.05）; After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly （P〈0.05 or P〈0.01）; Compared with the Benazepril group, QFTLObjective： To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription （祛风通络方, QFTL） on the regulation of mesangial cells （MCs） proliferation and apoptosis. Methods： The MCs used in this experiment have undergone five passages induced by lipopolysaccharide （LPS）. Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium （MTT） reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction （qRT-PCR）, respectively. Results： The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively （P〈0.05 or P〈0.01）. Moreover, the inhibitory effect is more significant in the QFTL group at 48 h （P〈0.05）. Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase （P〈0.05 or P〈0.01） to cells at S phase （P〈0.01）, implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group （P〈0.05）, while QFTL and Benazepril serum increased the level of MC apoptosis （P〈0.01）. Moreover, the difference between the QFTL group and the Benazepril group was statistically significant （P〈0.01）. Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 （CDK2） and p21 were significantly increased （P〈0.05 or P〈0.01）, p27 was decreased but with no statistical significance （P〉0.05）; After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly （P〈0.05 or P〈0.01）; Compared with the Benazepril group, QFTL

关 键 词：Qufeng Tongluo Prescription mesangial cells cell proliferation cell apoptosis cell cycle cell cycle regulatory proteins 

分 类 号：R692[医药卫生—泌尿科学]