右美托咪定联合浅低温对脑缺血损伤大鼠神经保护作用中炎症因子的影响  

Anti-Inflammatory Effect of Dexmedetomidine Combined with Mild Hypothermia on Cerebral Ischemia in Rats

作　　者：赵彬[1] 程江霞[1] 胡锦涛[1] 史艳燕[1] 彭晓红[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院麻醉科,湖北武汉430033

出　　处：《武汉大学学报（医学版）》2013年第5期654-656,共3页Medical Journal of Wuhan University

基　　金：武汉市卫生局资助项目[编号:武卫(2011)99]

摘　　要：目的:探讨右美托咪定联合浅低温对脑缺血损伤大鼠神经的保护作用。方法:雄性SD大鼠60只随机分为5组,假手术组(SH组),缺血损伤组(I组),浅低温组(T组),右美托咪定组(D组),浅低温复合右美托咪定(DT组)。建立大鼠脑缺血损伤模型,SH组仅分离出颈部血管不处理,同时维持颞肌温度约37.5℃;I组,于恒温箱中保持体温37.5℃,分离并结扎右颈总动脉和颈内动脉,60min后松开结扎线复灌;T组,保持体温约35℃,其余处理同I组;D组,保持体温约37.5℃,于结扎前30min腹腔注射右美托咪定100μg/kg,其余处理同I组;DT组,保持体温约35℃,其余处理同D组。术后72h对大鼠进行神经功能评分后,断头取脑采用干湿重法测量缺血脑组织含水量,用ELISA法检测血清中TNF-α,IL-6含量。结果:复灌72h结束后,T组、D组、DT组脑组织含水量较I组明显减少(P<0.05),T组、D组及DT组血清中TNF-α和IL-6含量较I组明显减少(P<0.05)。结论:右美托咪定联合浅低温对脑缺血损伤大鼠神经有保护作用。Objective： To investigate the protective effect of combination of mild hypothermia and dexmedetomidine on cerebral ischemia in rats. Methods. Sixty male SD rats were randomly divid- ed into five groups, with 12 rats in each group, sham-operated group （group SH）, ischemia group （group I）, mild hypothermia （group T）, Dexmedetomidine group （group D）, and Dexme- detomidine combined with mild hypothermia group （group DT）. Focal cerebral ischemia model was established by occluding the middle cerebral artery for 60 rain followed by 72 h reperfusion. Groups D and DT received intraperitoneal Dexmedetomidine 100 μg/kg 30 min before occlusion. Neurologic function was assessed and scored at the end of reperfusion. Then the rats were sacri- ficed, blood and brain were collected for determination of expression of cytokines and brain water content. Results.. Compared with group I, brain water content in groups T, D, and DT were significantly lower （P 〈 0. 05）, and expression of TNF-α and IL-6 were significantly lower. Conclusion： The combination of mild hypothermia and dexmedetomidine could alleviate cerebral ischemie injury in rats.

关 键 词：右美托咪定 浅低温 脑缺血损伤 

分 类 号：R614[医药卫生—麻醉学]