丹参酮ⅡA磺酸钠对大鼠肺成纤维细胞转化生长因子β_1信号通路的作用  

Effects of Sulfotanshinone Sodium on Transforming Growth Factor Beta 1 Signaling Pathway in Lung Fibroblasts of Rats

作　　者：殷静[1] 程真顺[1] 谭维军[1] 张莉[1] 谭秋月[1] 

机构地区：[1]武汉大学中南医院呼吸科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2013年第5期657-661,共5页Medical Journal of Wuhan University

摘　　要：目的:研究丹参酮ⅡA磺酸钠注射液(STS)对大鼠肺成纤维细胞转化生长因子β1(TGF-β1)Smad信号通路的影响,探讨STS抑制肺成纤维细胞(LFB)增殖、转化的可能机制。方法:体外分离培养大鼠肺成纤维细胞,实验分为3组:①空白对照组;②TGF-β1刺激组;③联合处理组:STS+TGF-β1刺激组,培养48h,待细胞生长同步化后,用MTT法检测丹参酮ⅡA对肺成纤维细胞增殖的影响,用RT-PCR法分析丹参酮ⅡA磺酸钠和TGF-β1作用后肺成纤维细胞Smad3、Smad7 mRNA表达水平的变化,以及对Ⅰ型胶原mRNA的影响。结果:丹参酮ⅡA磺酸钠在80-640mg/L浓度范围对5μg/L TGF-β1刺激的肺成纤维细胞增殖均具有明显的抑制作用,并呈剂量依赖性(P<0.05);丹参酮ⅡA磺酸钠在浓度160mg/L时能明显下调TGF-β1刺激的肺成纤维细胞内Smad3 mRNA、Smad3/Smad7 mRNA及Ⅰ型胶原mRNA的表达(P<0.05),并能上调Smad7 mRNA表达(P<0.05)。结论:丹参酮ⅡA磺酸钠能抑制LFB的增殖、转化及胶原的合成,机制可能是丹参酮ⅡA磺酸钠下调了大鼠TGF-β1 Smad信号通道的Smad3/Smad7水平,从而抑制LFB的增殖、转化。Objective： To study the effects of sulfotanshinone sodium （STS） on transforming growth factor beta 1 （TGF-~,） signaling pathway in lung fibroblasts of rats, and attempt to investigate its mechanism in prevention the proliferation and the transformation of lung fibroblasts. Methods： Rat lung fibroblasts were cultured in vitro and divided into three groups, blank control group, TGF-β1 stimulated group, and combined treatment group which was stimulated with STS and TGF-β1. After treatment for 48 hours, the mRNAs level of Smad3, Smad7, and collagen were assayed by reverse transcriptase polymerase chain reaction （RT-PCR）. Results： STS ranged 80-640 mg/L had obvious inhibitory effect on lung fibroblasts stimulated by 5 μg/L TGF-β1 in a dose-dependent manner （P〈0.05）, and 160 mg/L STS could down-regulate the mRNA levels of Smad3, Smad3/Smad7, and collagen type 0.05）, and could up-regulate the expression restrain the proliferation and transformation I of TGF-β1 stimulated lung fibroblasts （all P 〈 of Smad7 mRNA （P〈0.05）. Conclusion： STS can of lung fibroblasts, and synthesis of collagen. Themechanisms may be that STS can block TGF-β1 Smad signaling pathway and decrease the level of Smad3/SmadT.

关 键 词：丹参酮ⅡA 成纤维细胞 转化生长因子Β1 

分 类 号：R563.13[医药卫生—呼吸系统]