云南省鼠疫耶尔森菌脉冲场凝胶电泳分析  

Analysis of Yersinia pestis by pulsed-field gel electrophoresis in Yunnan Province

作　　者：王鹏[1] 石丽媛[2] 叶蕊[3] 崔志刚[4] 李伟[1] 杨光璨[2] 董珊珊[1] 郭英[2] 宋志忠[1] 

机构地区：[1]大理学院公共卫生学院、 [2]云南省地方病防治所云南省鼠疫防控技术重点实验室,大理671000 [3]大理学院公共卫生学院 [4]中国疾病预防控制中心传染病预防控制所

出　　处：《中华地方病学杂志》2013年第6期605-607,共3页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160354）;云南省应用基础研究（2011FB134）;卫生行业科研专项（201202021）

摘　　要：目的构建云南省鼠疫菌株脉冲场凝胶电泳（PFGE）图谱库，为云南鼠疫的溯源及分型防控提供依据。方法选取云南省家、野鼠型以及玉龙鼠疫菌株共29株，分别采用AscI和FseI两种内切酶对菌株进行酶切分析，并使用BioNumerics6．6软件对图谱聚类。结果PFGE分析可以将29株鼠疫菌分为20个基因型，这些基因型又归为3大簇，即家鼠基因簇、野鼠基因簇以及玉龙基因簇，家鼠基因簇又可分为德宏亚簇、文山亚簇及临沧亚簇。结论云南鼠疫菌PFGE分子分型具有区域聚集性；PFGE基因型与菌株生态型能够很好地对应；玉龙鼠疫菌是云南一个独立的PFGE基因型。Objective To construct pulsed-field gel electrophoresis （PFGE） library typing of Yersinia pestis in Yunnan Province, and to provide a basis for control of the disease through originating and typing the disease. Methods Twenty-nine strains isolated from Rattus flavipectus plague focus, Apodemus chevrieri- Eothenomys miletus plague focus and Yulong plague focus in Yunnan Province were involved in this study. Two kinds of endonucleases of Asc I and Fse I were used in PFGE analysis, and clustering patterns were draw using BioNumerics 6.6 software. Results The 29 strains of Yersinia pestis can be divided into 20 genotypes by PFGE. These genotypes were grouped into three major clusters, namely Rattus flavipectus gene cluster, Apodemus chevrieri-Eothenomys miletus gene cluster and Yulong gene cluster. Rattus flavipectus gene cluster could be further divided into 3 sub-clusters of Dehong, Wenshan and Lincang. Conclusions There are regional aggregations of molecular typing of Yersinia pestis in Yunnan Province by PFGE; and the result of PFGE genotyping is well related to that of ecological typing; Yulong plague is an independent PFGE pattern.

关 键 词：耶尔森菌 鼠疫 电泳 凝胶 脉冲场 云南 

分 类 号：R378[医药卫生—病原生物学]