慢性血吸虫病患者结肠腺咬合蛋白与紧密连接蛋白-1的表达变化  

Expression of occludin and zonula occludens.1 in colonic gland of patients with chronic schistosomiasis

作　　者：冯林森[1] 李靖娟[1] 张爱芬[1] 吴皎[1] 吴泽生[2] 张东葵[3] 段艳萍[1] 王金德[4] 

机构地区：[1]昆明医科大学临床医学年级,650500 [2]大理学院附属医院消化内科 [3]昆明医科大学形态学实验室 [4]大理学院附属医院人体解剖学教研室、研究生部

出　　处：《中华地方病学杂志》2013年第6期612-615,共4页Chinese Journal of Endemiology

基　　金：云南省教育科学规划（GY09006）;2012年地方高校国家级大学生创新创业训练计划（201210678002）;2013年度昆明医科大学大学生创新性实验计划（CX20870）

摘　　要：目的探讨慢性血吸虫病患者结肠腺咬合蛋白（occludin）与紧密连接蛋白-1（ZO—1）的表达及其临床意义。方法选取50例慢性血吸虫病患者及9例正常结肠黏膜标本，采用免疫组织化学（SABC）染色法，检测occludin与ZO-1在结肠腺上皮细胞的表达情况。结果与对照组比较，慢性血吸虫病组结肠腺结构不完整、腺体萎缩；慢性血吸虫病组结肠腺上皮细胞occludin与ZO-1阳性颗粒平均灰度值（159．26±6．57、166．07±5．44）明显高于对照组（155．53±5．90、162．16±5．14，P〈0．05），阳性单位值（37．27±2．63、34．69±2．19）明显低于对照组（38．85±2．42、36．37±2．11，P〈0．05）；慢性血吸虫病组结肠腺occludin与ZO-1表达量的减少与性别及年龄无关（P〉0．05）。结论血吸虫感染可以损伤结肠腺上皮细胞间的紧密连接蛋白，从而破坏患者结肠黏膜屏障的完整性。Objective To study the expression of occludin and zonula occludens-1 （ZO-1） in patients with chronic schistosomiasis and its clinical significance. Methods Colonic mucosa specimens of 50 patients with chronic schistosomiasis and 9 cases of normal people （as a control group） were selected and the expressions of occludin and ZO：I in colonic epithelial cells were determined using immunohistochemical staining（SABC）. Results Compared with the normal control group, the structure of colonic gland was incomplete with glandular atrophy, mean gray values（159.26 ~ 6.57, 166.07 ~ 5.44） of the positive particles of oceludin and ZO-1 of colonic epithelial cells in the chronic schistosomiasis group were significantly higher than those of the normal control group（155.53 ~ 5.90, 162.16 ~ 5.14, P 〈 0.05）, but positive unit values（37.27 ~ 2.63, 34.69 ~ 2.19） were significantly lower than those of the control group（38.85 ~ 2.42, 36.37 ~ 2.11, P 〈 0.05）; reduced expression of oecludin and ZO-1 in colonic gland of the patients with chronic schistosomiasis had nothing to do with sex or age （P 〉 0.05）. Conclusions Infection of schistosome can reduce the expression of oecludin and ZO-1 which can damage the tight junction between colonic epithelium cells, thereby undermining the integrity of the colonic mueosa barrier.

关 键 词：慢性血吸虫病 结肠腺 咬合蛋白 紧密连接蛋白-1 

分 类 号：R532.2[医药卫生—内科学]