饮食低硒对小鼠肝、肾、脑组织硒结合蛋白1与硒蛋白P表达的影响  

Influence of low-selenium diet on expression of selenium-binding protein 1 and selenoprotein P in mouse liver, kidney and brain tissues

作　　者：孙美娜[1] 赵汉东[1] 张一彤[1] 李晖[1] 

机构地区：[1]哈尔滨医科大学基础医学院生物化学与分子生物学教研室,150081

出　　处：《中华地方病学杂志》2013年第6期636-638,共3页Chinese Journal of Endemiology

基　　金：国家自然科学基金（30771863、81172616）;黑龙江省大学生创新创业训练计划项目（201210226024）

摘　　要：目的探讨饮食低硒对小鼠肝、肾、脑组织硒结合蛋白1（SBP1）和硒蛋白P（SelP）表达的影响。方法将C57BL／6小鼠按体质量随机分为4组：对照组和低硒4、12、24周组，每组10只，雌雄各半。对照组饲以普通饲料（含硒量为0．300mg／kg），饮用蒸馏水，第12周断颈处死；低硒各组饲以低硒饲料（含硒量为0．015mg／kg），分别于第4、12、24周时断颈处死。采用蛋白印迹（Western blot）法检测小鼠肝、肾、脑组织SBPl与SelP蛋白表达。结果低硒4、12、24周组小鼠肝组织中SBP1和SelP蛋白表达分别为0．11±0．01、0．36±0．01、0．59±0．02和0．41±0．01、0．39±0．02、0．25±0．02；肾脏组织中分别为0．60±0．03、0．20±0．02、0．03±0．01和O．88±0．01、0．73±0．03、0．85±0．02；脑组织中分别为0．54±0．03、0．11±0．01、0．01±0．01和0．50±0．02、0．49±0．03、0．38±0．02。低硒各组小鼠肝脏、肾脏、脑组织SBP1、SelP蛋白表达与对照组（1．00±0．00、1．00±0．00）比较明显降低（P均〈0．05），其中各脏器中SBPI的表达量随低硒喂养时间的延长各有不同．肝脏先降低后回升，肾脏和脑组织则持续降低。结论低硒饮食对小鼠肝、肾、脑组织内SelP和SBP1的表达量有一定的影响．Objective To explore the influence of low-selenium diet on expression of selenium-binding protein 1 （SBP1） and selenoprotein P（Sel P） in liver, kidney and brain tissues. Methods C57BL/6 mice were randomly divided into four groups according to body weight： control group, low-selenium treatment for 4-, 12- and 24-week groups, 10 mice in each group, half male and half female. The control group was fed with normal diet （selenium content was 0.300 mg/kg）, distilled water, and sacrificed at the 12th week; low-selenium treatment groups were fed with low-selenium diet（selenium content was 0.015 mg/kg）, then sacrificed at the 4th, 12th and 24th weeks, respectively. Expressions of SBP1 and Sel P in mouse liver, kidney and brain tissues were determined by Western blotting. Results Expressions of SBP1 and Sel P in low-selenium feed mouse liver tissue at the 4th, 12th and 24th weeks were, respectively, as follows 0.11±0.01, 0.36 ± 0.01, 0.59 ± 0.02 and 0.41 ± 0.01, 0.39 ± 0.02, 0.25 ± 0.02;in kidney, respectively, as follows 0.60 ± 0.03, 0.20 ± 0.02, 0.03 ± 0.01 and 0.88 ± 0.01, 0.73 ± 0.03, 0.85 ± 0.02; in brain, respectively, as follows 0.54 ± 0.03, 0.11 ± 0.01, 0.01 ± 0.01 and 0.50 ± 0.02, 0.49 ± 0.03, 0.38 ± 0.02. Expression of Sel P in low-selenium feed mouse liver, kidney and brain tissues was significantly decreased as compared to that of control group（1.00 ± 0.00, 1.00 ± 0.00, all P 〈 0.05）, but SBP1 content was reduced at first and then rebounded in kidney, and was in decreasing trend in liver and brain tissues. Conclusion Low-selenium diet has a certain effect on expression of SBP1 and Sel P in mouse liver, kidney and brain tissues.

关 键 词：硒 硒结合蛋白1 硒蛋白P 

分 类 号：R341[医药卫生—基础医学]