苦参素对实验性自身免疫性脑脊髓炎大鼠Nogo-A/NgR表达的影响  

Effect of matrine on Nogo-A/NgR of experimental autoimmune encephalomyetic rats

作　　者：张夙[1,2] 朱琳[1,2] 吕莹[3] 楚尧娟[1,2] 吕鹏[1,2] 张广先 

机构地区：[1]郑州大学第一附属医院 [2]河南省高等学校临床医学重点学科开放实验室,郑州450000 [3]郑州大学,郑州450000 [4]托马斯杰弗逊大学

出　　处：《中药药理与临床》2013年第5期30-33,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省科技厅2012科技发展计划项目;编号:122102310229

摘　　要：目的:观察苦参素对实验性自身免疫性脑脊髓炎(EAE)大鼠脊髓中Nogo-A及其受体NgR表达水平的影响,探讨苦参素对EAE大鼠的防治机制。方法:用豚鼠全脊髓匀浆和完全弗氏佐剂制成的抗原乳剂复制备EAE模型,将50只大鼠随机分为正常组、模型组、苦参素低、高剂量组(150,200mg/kg)、地塞米松组(1mg/kg),连续腹腔注射给药16天,同期模型组和正常组腹腔注射等量生理盐水,每天观察记录大鼠体重变化,神经功能学评分,苏木素-伊红(HE)和变色酸2R-亮绿(C-2R-BG)染色观察病理改变、免疫组织化学法检测脊髓中Nogo-A的表达、RT-PCR法测定脊髓中NgR含量的变化。结果:苦参素(150、200mg/kg)能够明显推迟EAE大鼠发病时间,降低EAE大鼠神经功能评分,改善EAE导致的大鼠体重减轻,抑制EAE大鼠中枢神经系统炎症浸润和髓鞘脱失,下调脊髓中Nogo-A和NgR的表达,并且高低剂量组间存在统计学差异。结论:苦参素对EAE大鼠具有防治作用,其机制可能与下调脊髓中Nogo-A和NgR的表达有关。We observed the effect of matrine on the expression of Nogo-A and NgR in experimental autoimmune encephalomyetic （EAE） rats and investigated the possible mechanism of matrine for the prevention and treatment of EAE rats. Methods： The EAE models were induced by immuned spinal cord extracts of guinea pig with complete Freunds＇adjuvant. Then we divided 50 Wistar rats into five groups randomly： normal group, model group, two matfine-treated groups （ 150,200mg/kg）, dexamethasone-treated group. Rats of MAT-treated groups and DEX-treated group were injected intraperitoneally with MAT and DEX daily for 16 days respectively,whereas rats of normal group and model group were injected intraperitoneally with normal saline. We also observed body weights,clinical signs daily. Histopathological e valuation of spinal cord was analyzed by hematoxylin-eosin （HE） and chromotrope 2R-brilliant green （C-2R-BG）. Nogo-A and NgR in spi nal cord were determined using immunohistochemistry and RT-PCR respectively. Results： Matrine treatment significantly putted off the mor bidity,decreased neurological scores and weight loss after EAE. MAT-treated rats showed a significant decrease in CNS infiltration of inflam- matory cells and demyelination. Furthermore, the expression of Nogo-A and NgR in the spinal cord showed a marked reduction after MAT treatment, particularly in rats treated with higher doses of MAT. Conclusion： Matrine might be a new and safe therapy for EAE, as well as the mechanism is related to reduce the expression of Nogo-A and NgR.

关 键 词：苦参素 实验性自身免疫性脑脊髓炎 NOGO-A NGR 

分 类 号：R285[医药卫生—中药学]