葛根素抗ApoE^(-/-)小鼠动脉粥样硬化的炎症机制研究  

The inflammatory mechanism of puerarin anti-atherosclerosis in ApoE knockout mice

作　　者：周凤华[1] 黄志勇[2] 娄林洁[3] 余林中[1] 陈育尧[1] 刁建新[1] 崔小冰[1] 万强[1] 贾钰华[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]中国人民解放军空军雷达学院卫生处,武汉430019 [3]温州医学院第一附属医院中医科,温州325000

出　　处：《中药药理与临床》2013年第5期33-36,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(项目编号:81072776)

摘　　要：目的:观察葛根素对ApoE基因敲除小鼠炎症因子的影响,探讨其是否能抗AS炎症反应;方法:将24只ApoE-/-小鼠随机分为模型组、葛根素组、辛伐他汀组,每组8只,另取8只C57BL/6J小鼠作为正常对照组。采用高脂喂养16周的方式建立AS模型,分别以胸主动脉与主动脉窦部位切片观察主动脉斑块形态,荧光定量PCR法检测小鼠主动脉斑块内IL-2、IL-6、TNF-α、hsCRP等炎症因子mRNA表达;结果:与正常对照组相比,模型组小鼠血脂与炎症因子mRNA水平明显增高,主动脉内有纤维斑块形成;辛伐他汀(5mg/kg)与葛根素(65mg/kg)均能明显降低炎症因子mRNA表达,并且能抑制斑块形成,葛根素效果更佳。结论:葛根素能降低AS斑块炎症因子的表达,抗炎机制可能是其抗AS的其中一个药理机制。To study whether puerarin can anti-inflammatory response in atheroscleresis; Methods ：24 ApoE^-/- mice were randomly divided into model group, puerarin group, simvastatin group （n =8）, another 8 C57BL/6J mice served as normal group. High fat diet was giving to ApoE/ mice for 16 weeks to establish AS model. The thoracic aorta and aortic sinus was sliced to observe aortic plaque morphology; Quanti tative PCR assay was used to detect IL-2, IL-6, TNF-^1, hs-CRP mRNA in mouse aortic; Results：compared with the normal control group, the mRNA levels of inflammatory cytokines and blood lipids in model mice was significantly higher （ P 〈 0.01 ）, and there was intra-aortic fi brous plaque formation; Sim atorvastatin and puerarin could significantly not only reduce the inflammatory cytokine mRNA expression （ P 〈 0. 01 ）, but also inhibit plaque formation, especially in puerarin group; Condusion：Puerarin can reduce the expression of inflammatory factors in AS plaque, which may be one of pharmacological mechanisms of puerarin anti-atherosclerosis.

关 键 词：葛根素 动脉粥样硬化 APOE 炎症 

分 类 号：R285[医药卫生—中药学]