茯苓多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响  

Effect of pachymaran on humoral immune function in immunosuppressed mice induced by cyclophosphamide

作　　者：彭小彬[1] 邱小惠[1] 余传林[1] 陈娜娜[1] 雷林生[1] 

机构地区：[1]南方医科大学药学院,广州510515

出　　处：《中药药理与临床》2013年第5期69-72,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察茯苓多糖能否缓解环磷酰胺对小鼠体液免疫功能的抑制作用,并初步探讨其作用机制。方法:灌胃正常和环磷酰胺处理小鼠茯苓多糖,生理盐水作为对照,每天1次,于第11天取血,ELISA测血清IgG、IgM。动物在第6天给小鼠腹腔注射20%的兔红细胞0.2ml,分光光度法测血清溶血素,ELISA测血清IL-4,实时定量PCR测脾脏IL-4 mRNA。分离正常和环磷酰胺处理小鼠脾细胞,体外施加茯苓多糖,培养48小时,ELISA测上清IgG、IgM。结果:剂量为100mg/kg的茯苓多糖对正常小鼠和剂量为100及50mg/kg的茯苓多糖对环磷酰胺免疫抑制小鼠血清天然IgG和IgM水平以及溶血素、IL-4、脾脏IL-4 mRNA水平均有提升作用,对环磷酰胺模型小鼠的最小有效剂量为50mg/kg;以100mg/kg的剂量为准进行比较,茯苓多糖对环磷酰胺模型小鼠效果更佳。另外,浓度为50及25μg/ml的茯苓多糖对环磷酰胺处理的小鼠脾细胞产生IgG和IgM也有促进作用,最低有效浓度为25μg/ml。结论:茯苓多糖可以缓解环磷酰胺对小鼠体液免疫功能的抑制作用。To determine if pachymaran can alleviate suppression of humoral immune function in mice induced by cyclophosphamide and to preliminarily explore the underlying mechanism. Methods： Experiment 1, normal and cyclophosphamide-treated mice were gastrically ad ministered pachymaran or saline as control once a day. Serum samples were collected on day 11 and used for assay of IgG and IgM by ELISA. Experiment 2, the animals were treated as the same as described in Experiment 1 except that each of the animals was additionally intraperito neally injected with 0.2ml of 20% rabbit red blood cells. Serum hemolysin, IL-4, and spleen IL-4 mRNA were determined by spectropho tometry, ELISA and quantitative real time PCR, respectively. And experiment 3, splenocytes from normal and cyclophosphamide-treated mice were prepared and cultured in the presence or absence of pachymaran in vitro for 48 h. The amounts of IgG and IgM in the supematants were assessed by ELISA. Results： Treatment of normal mice with pachymaran in a dose of 100mg/kg and cyclophosphamide-treated mice in doses of 100 and 50mg/kg increased the levels of serum natural IgG and IgM, hemolysin, IL-4, and spleen IL-4 mRNA. The minimal effec tive dose was observed at 50 mg/kg in cyclophosphamide-treated mice and the potentiating effect of pachymaran was more potent in cyclophos phamide-treated mice than in normal ones when compared by the extent of potentiation at the dose of 100mg/kg. In vitro study revealed that pachymaran at concentrations of S0 and 25μg/ml could also enhance production of IgG and IgM by splenecytes from cyclophosphamide-treated mice, with a minimal effective concentration of 25μg/ml. Conclusion： Pachymaran can alleviate cyclophosphamide-induced suppression of humoral immune function in mice.

关 键 词：茯苓多糖 环磷酰胺 IgG IGM 溶血素 白介素4 

分 类 号：R285[医药卫生—中药学]