复方菊明对大鼠离体胸主动脉环收缩效应的影响  

Effect of compound flos Chrysanthemi indici and Semen cassiae in contracting isolated thoracic aorta rings in the rats

作　　者：庞敏霞[1] 余芳[1] 陈素红[2] 苏洁[1] 陈琦[1] 吕圭源[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]温州医学院,温州325035

出　　处：《中药药理与临床》2013年第5期118-121,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(81274123);国家"重大新药创制"科技重大专项(2011ZX09101-002-07;2009ZX09502-016);浙江省卫生高层次创新人才培养工程项目(浙卫发[2010]190号)

摘　　要：目的:观察复方菊明对大鼠离体胸主动脉环收缩作用的影响。方法:采用离体血管张力实验法,观察复方菊明、维拉帕米(Ver)和生理盐水对NA、高K+液引起的主动脉环收缩的影响,以及对NA诱导的主动脉环依赖细胞内钙与外钙收缩的影响。结果:复方菊明(4.0mg/ml^8.0mg/ml)和Ver能拮抗NA(2.37μmol/l)和KCl(2.37μmol/l)诱导的主动脉环收缩作用,生理盐水无影响。在相同的浓度下,复方菊明拮抗NA诱导的主动脉环收缩作用比对KCl诱导的主动脉环收缩的拮抗作用强。此外,复方菊明(5.0mg/ml)对NA诱导的依赖细胞内钙、外钙的收缩均有抑制,Ver对NA诱导的依赖细胞内钙的收缩有抑制作用、对依赖细胞外钙的收缩没有影响;生理盐水组给药前后没有变化。结论:复方菊明具有直接舒张血管作用,能阻断受体依赖性钙通道(ROC)和电压依赖性钙通道(VDC),其降压机制可能通过阻断ROC,抑制内钙释放外钙内流,从而降低胞内Ca2+浓度,发挥降压作用。To investigate the effect of Compound flos Chrysanthemi indici and Semen eassiae （FJM） in contracting isolated thoracic a orta tings in the rats. Method： Isolated vascular tension experiment were operated to respectively observe the effect of FJM, Ver, NS on aorta rings contraction induced by NA and high level of K ＋. Also, study its effect on intracellular and extracellular Ca^2＋ -dependent aorta rings contraction, induced by NA. Result： FJM（4.0mg/ml ～ 8.0mg/ml）and Ver could inhibit the contraction of aortic ring evoked by NA（2. 37 μmol/l）or KCI （40mmol/1） （P 〈 0.01 ）, NS has no inhibition. In the same concentrations, the inhibition effect of aortic ring evoked by NA is more obvious than that evoked by KC1. FJM （5.0mg/ml）could also inhibit intraeellular and extracellular Ca^2＋ -dependent contraction, induced by NA（P 〈0.01 ）. Ver（5 x 10-3 mg/ml）could inhibit intracellular Ca^2＋-dependem contraction, induced by NA. NS shows no change. Conclusion： FJM could directly constrict blood vessels. Inhibition of receptor operated calcium channel（ROC） and voltage-depend ent calcium channel（VDC）were also observed. The mechanism of antihypertensive possibly due to block ROC and inhibit internal Ca^2＋ re- lease and external Ca^2 ＋ influx, therefore reduce Ca^2＋ concentration in the cell.

关 键 词：复方菊明 胸主动脉环 收缩 

分 类 号：R285[医药卫生—中药学]