生物素-链霉亲和素技术一步法检测淋球菌抗原的研究  

Detection of Neisseria Gonorrhoeae Antigen by an One-step Enzyme Immunoassay Coupling with Biotin-streptavidin Technique

作　　者：沈建根[1] 朱永良[2] 董玉娥[3] 

机构地区：[1]浙江大学医学院,浙江杭州310031 [2]浙江大学附属第二医院,浙江杭州310009 [3]浙江省人民医院,浙江杭州310014

出　　处：《浙江大学学报（医学版）》2000年第6期250-252,共3页Journal of Zhejiang University(Medical Sciences)

基　　金：:浙江省卫生厅资助

摘　　要：目的 :建立一种灵敏、快速诊断淋球菌感染的酶免疫测定法。方法 :检测淋球菌抗原的生物素 -链霉亲和素酶联免疫一步法。结果 :36例临床淋球菌标本中淋球菌抗原含量 1 0 8.47± 5 1 .39μg/ L ,阳性率 97.2 % ,显著高于非淋球菌标本的 4.71± 2 .1 3μg/ L ,阳性率 0 % (P均 <0 .0 1 ) ,本法检测灵敏度 0 .6 2 5 μg/ L,特异性 1 0 0 % ,敏感性 97.2 %。结论 :该法可作为灵敏、快速诊断淋球菌的新的辅助方法。Objective:To establish a sensitive and rapid diagnostic method of N.gonorrhoeae infection.Methods:An one step enzyme immunoassay with biotin streptavidin for N.gonorrhoeae antigens.Results:The N.gonorrhoeae antigen content in 36 clinical N.gonorrhoeae samples was 108.47±51.39 μg/L and the positive rates was 97.2%,Both values were higher than that in non N.gonorrhoeae samples ( P <0.01).Conclusion:This method could be used as a new sensitive and rapid diagnostic technique for N.gonorrhoeae infection.

关 键 词：淋病奈瑟氏球菌 单克降抗体 生物素-链霉亲和素 

分 类 号：R446.5[医药卫生—诊断学]