Ly294002对人白血病HL-60细胞的体外抑制作用  

Inhibition of phosphatidylinositol 3-kinase inhibitor Ly294002 to leukaemia HL-60 cells

作　　者：王迪迪[1] 葛堂栋[1] 杨玉[1] 张鹏霞[1] 

机构地区：[1]佳木斯大学基础医学院生物化学教研室,黑龙江佳木斯154007

出　　处：《中国老年学杂志》2013年第22期5617-5619,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81070428);黑龙江省卫生厅科研课题(No.2011-438);佳木斯大学科研项目(No.s2010-102)

摘　　要：目的研究磷脂酰肌醇-3激酶(PI3K)抑制剂Ly294002对人白血病HL-60细胞的影响。方法 MTT实验检测Ly294002对HL-60细胞增殖的抑制作用;透射电镜进行细胞形态学观察;RT-PCR检测Ly294002作用后,PI3K、Akt mRNA的表达。结果与未用药组比较Ly294002对HL-60细胞增殖具有抑制作用,且在24 h内随着作用浓度的增加而增强;透射电镜观察细胞出现凋亡形态学改变;RT-PCR检测用药组PI3K、Akt mRNA表达均下调(P<0.01)。结论 PI3K抑制剂Ly294002能通过PI3K/Akt信号通路抑制HL-60细胞增殖,50μmol/L的Ly294002作用白血病HL-60细胞24 h,其增殖抑制作用效果最佳。Objective To study the effect of Ly294002,an inhibitor of phosphatidylinositol 3-kinase, on the expression of survival of human leukaemia HL-60 cells. Methods The growth-inhibiting effects of P13K inhibitor（Ly294002） on HL-60 cells were detected by MTF assay; After being treated by drug, the morphological and structural changes of the cells were observed by transmission electronic microscopy ; the mRNA expressions of PI3K and Akt were measured by RT-PCR. Results In HL-60 cells, after being treated by Ly294002, the mRNA expression of PI3 K and Akt were down regulated （ P 〈 0. 01 ） by RT-PCR test. Conclusions PI3 K inhibitor （ Ly294002 ） could inhibit PI3K/Akt signal pathway, resulting in inhibiting cell proliferation in HL-60 cells. After being treated by 50 μmol/l Ly294002 in 24 h, the growth-inhibiting effects on HL-60 cells are the best.

关 键 词：白血病 LY294002 PI3K AKT HL-60 

分 类 号：R285.5[医药卫生—中药学] R741.02[医药卫生—中医学]