谷氨酰胺对慢性阻塞性肺疾病患者外周血单个核细胞中核因子кB及肿瘤坏死因子α表达的影响  

Effect of glutamine on expression of nuclear factor-κB and tumor necrosis factor-α in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease

作　　者：周凌燕[1,2] 黄美健[2] 汤晓燕[2] 居建刚[2] 冷报浪[2] 任娥[2] 

机构地区：[1]湖州市中心医院呼吸科,浙江湖州313000 [2]杭州市第三人民医院呼吸科,浙江杭州310009

出　　处：《中国新药与临床杂志》2013年第11期918-920,共3页Chinese Journal of New Drugs and Clinical Remedies

基　　金：杭州市卫生局项目(2011B009)

摘　　要：目的探讨谷氨酰胺(Gln)对慢性阻塞性肺疾病(COPD)患者外周血单个核细胞(PBMC)中核因子κB(NF-κB)和肿瘤坏死因子α(TNF-α)表达的影响。方法选择30例COPD急性加重(AECOPD)经治疗转为稳定期(SCOPD)的患者为研究对象,取其急性期和稳定期静脉血,分离PBMC。PBMC分为AECOPD组(空白对照)、AECOPD+Gln组、SCOPD组(空白对照)和SCOPD+Gln组,Gln终浓度为8 mmol·L^(-1)。另以15例健康体检者PBMC为正常对照组。Real-time PCR法检测五组PBMC中NF-κBp65 mRNA和TNF-αmRNA的表达水平,比较各组△Ct值。结果 AECOPD组和SCOPD组NF-κBp65、TNF-α的△Ct值均低于正常对照组(P<0.05或P<0.01),且AECOPD组低于SCOPD组(9.16±0.45 vs.9.80±0.52,8.81±1.17 vs.9.57±1.23,P<0.05)。SCOPD+Gln组NF-κBp65和TNF-α的△Ct值(10.36±0.53和11.21±1.38)高于SCOPD组,AECOPD+Gln组(10.04±0.75和10.58±1.22)高于AECOPD组,差异均非常显著(P<0.01)。结论 Gln可能通过抑制COPD患者NF-κB的活化,降低TNF-α的表达,从而达到抗炎作用。AIM To study the effect of glutamine （Gln） on the expression of nuclear factor factor-κB （NF- κB） and tumor necrosis factor- ct （TNF- α） in peripheral blood mononuclear cells （PBMC） in patients with chronic obstructive pulmonary disease （COPD） . METHODS Thirty patients with acute exacerbation of COPD （AECOPD） were enrolled. After treatment, all patients＇ conditions were controlled in stable period of COPD （SCOPD）. Venous blood of patients was drawn in the acute phase and stable period, and κBMC were extracted. There were four experiment groups in present study, including AECOPD group （AECOPD blank control）, AECOPD ＋Gln group, SCOPD group （SCOPD blank control）, SCOPD ＋ Gln group. The final concentration of Gln is 8 mmol·L-1. PBMC from 15 healthy people were used as normal group. The levels of NF- κBp65 and TNF- α gene expression were detected using real- time PCR, and △Ct values were calculated. RESULTS The △Ct values of NF-κBp65 and TNF-α were lower in AECOPD group and SCOPD group than those in the normal group （P 〈 0.05 and P 〈 0.01, respectively）. Moreover, there was a significantly reduction in both NF-κBp65 and TNF-α gene expression in AECOPD group compared with SCOPD group （9.16 ± 0.45 vs. 9.80± 0.52, P 〈 0.05 and 8.81 ± 1.17 vs. 9.57 ± 1.23, P 〈 0.05, respectively）. The ACt values of NF- κBp65 and TNF-α in the SCOPD ＋ Gln group were significantly higher than those in SCOPD group （10.36 ± 0.53 vs. 9.80 ± 0.52, P 〈 0.01 and 11.21 ± 1.38 vs. 9.57 ± 1.23, P 〈 0.01, respectively）. This upregulation was also found in AECOPD ＋Gln group compared with the AECOPD group （10.04 ± 0.75 vs. 9.16 ± 0.45, P 〈 0.01 and 10.58 ±1.22 vs. 8.81 ± 1.17, P 〈 0.01, respectively） . CONCLUSION Gln plays an anti-inflammatory role on COPD by inhibiting NF-κB activity and reducing TNF-α expression.

关 键 词：谷氨酰胺 肺疾病 慢性阻塞性 NF-кB 肿瘤坏死因子Α 单个核细胞 

分 类 号：R563[医药卫生—呼吸系统]