铕掺杂氧化钆纳米颗粒的核磁共振成像与荧光标记应用探究  

Europium doped gadolinium oxide nanoparticles for magnetic resonance and fluorescence imaging application

作　　者：杨川[1] 万雨[2,3] 冯英[1] 李幽兰[1] 谢富康[1] 李立[4] 

机构地区：[1]中山大学中山医学院人体解剖与组织胚胎学教研室,广东广州510080 [2]南方医科大学 [3]广州军区总医院整形外科 [4]中山大学肿瘤防治中心影像介入科

出　　处：《解剖学研究》2013年第5期364-367,379,共5页Anatomy Research

基　　金：国家自然科学资金(81271622;81071207)

摘　　要：目的制备双模态造影剂铕掺杂氧化钆(Gd2O3∶Eu3+)纳米颗粒,探究其应用于核磁共振成像(MRI)及荧光标记的可行性。方法通过液体环境激光烧蚀固体Gd2O3∶Eu3+靶材,得到Gd2O3∶Eu3+胶体纳米颗粒,通过透射电镜观察该纳米材料形貌特征;S18细胞增殖MTT实验表征其生物毒性;3.0T磁共振成像系统观察其在体外和Balb/c裸鼠体内鼻咽癌S18裸鼠移植瘤的显像特点;荧光显微镜观察其在细胞内荧光标记效果。结果液体环境激光熔蚀技术制备的纳米颗粒分散性良好,平均粒径为7.2 nm;材料生物毒性低;体外MRI成像呈明显梯度;将该材料尾静脉注射裸鼠后约30 min,鼻咽癌S18裸鼠移植瘤信号强度逐渐增高;细胞与纳米颗粒共同培养4 h后,荧光显微镜显示纳米颗粒被细胞吞噬并发出红色荧光。结论成功研制了分散性良好,生物毒性低的双模态造影剂Gd2O3∶Eu3+,并将其应用与小鼠体内MRI成像和细胞荧光成像,得到了良好的成像效果,为结合MRI与荧光成像技术提供一种途径。Objective To prepare europium doped gadolinium oxide （Gd203：Eu3＋） nanoparticles for use as potential mag- netic resonace imaging （MRI） and fluorescence imaging dualmodal contrast agents. Methods Prepare Gd203：Eu3＋ nanoparticles by laser ablation in liquid （LAL） whose mophology was chareacterized by TEM. Cell viability of S18 cells was used to evaluate the bio- compatibility of the nanopaticles. In vitro and in vivo MRI were carried out by a 3.0T Siemens Trio MRI scanner, while the S18 cells fluorescence imaging were analysed by fluorescence microscope （Olympus IX51, Janpan）. Results These nanoparticles ob- tain excellent dispersibility and good biocompatibility. In vitro and in vivo MRI show them to be suitable MR1 contrast agents. Satis- factory S18 fluorescence images were also observed. Conclusion Gd203：Eu3＋ nanoparticles of great dispersibility and biocompati- bility are successfully synthesized by LAL. These nanoparticles show good ability in MRI and fluorescence imaging and provide a strategy to combine these two clinical imaging techniques.

关 键 词：核磁共振成像 荧光成像 液体环境激光熔蚀 鼻咽癌 

分 类 号：R445.2[医药卫生—影像医学与核医学]