曲古霉素A对小鼠骨髓源性树突状细胞表型和功能的影响  

Effects of Trichostatin A on the phenotype and function of mouse bone marrow-derived dendritic cells

作　　者：江涛[1] 刘牧林[1] 张宗兵[1] 孔令尚[1] 

机构地区：[1]蚌埠医学院第一附属医院胃肠外科,蚌埠医学硕士研究生233004

出　　处：《医学研究生学报》2013年第11期1152-1155,共4页Journal of Medical Postgraduates

摘　　要：目的未成熟树突状细胞(immature dendritic cells,imDCs)诱导免疫耐受的作用受到越来越多的重视,如何保持其未成熟状态成为研究热点。文中旨在研究曲古霉素A(Trichostatin A,TSA)对小鼠骨髓源性树突状细胞(dendritic cells,DCs)表型和功能的影响。方法取小鼠骨髓单核细胞,在含重组小鼠粒/巨细胞刺激因子(recombinant mouse granulocytemacrophage colony-stimulating factor,rmGM-CSF)和重组小鼠白介素-4(recombinant mouse interleukin 4,rmIL-4)完全培养基中诱导其向DCs分化,并与第6天收集疏松贴壁细胞分为空白组(不另外添加任何药物)和TAS组(加入100ng/ml)组,分别继续培养48 h。流式细胞仪测定各组DCs表面共刺激因子的表达,ELISA试剂盒检测IL-12p40细胞因子分泌的变化,混合淋巴细胞增殖实验检测不同组DCs对T淋巴细胞增殖能力的影响。结果与空白组比较,TSA显著下调DCs表面共刺激因子的表达和减少细胞因子IL-12p40的分泌,且其诱导的DCs显著降低同种异基因T淋巴细胞增殖能力。结论 TSA能够抑制DCs的成熟和诱导imDCs的产生。Objective The immature dendritic cells （DC） can induce immunological tolerance and how to maintain their im- mature state is becoming a focus of research. The aim of this study is to investigate the effect of Triehostatin A （TSA） on the phenotype and function of mouse bone marrow-derived dendritic ceils. Methods We isolated mononuelear cells from mouse bone marrow and in- duced their differentiation into DCs by culturing them with recombinant mouse granuloeyte-maerophage colony-stimulating factor （rmGM- CSF） and recombinant mouse interleakin 4 （rmlL-4） for 6 days. Then we randomized the cells into a blank control and a TSA group and again cultured them for 48 hours, the latter with TSA at 1130 ng/ml. We detected the expressions of the DC surface costimulating factors by flow cytometry, observed the changes in the concentration of supernatant IL-12p40 by ELISA, and determined the influence of DCs on the proliferation of T-cells by MTF. Results Compared with the blank control, TSA remarkably down-regulated the expressions of the DC surface costimulating factors and decreased the secretion of interleukin-12p40, and the TSA-treated DCs markedly reduced the prolif- eration of T-cells. Conclusion TSA can inhibit the maturation of dendritic cells and induce the production of immature dendritic cells.

关 键 词：曲古霉素A 树突状细胞 混合淋巴细胞反应 免疫功能 

分 类 号：R978.5[医药卫生—药品]