流动注射电化学发光测定动物饲料中6-丙基-2-硫脲嘧啶含量的研究  

Determination of 6-Propy-2-thiouracile in Animal Feeds by Flow-injection Electrochemiluminescence Method

作　　者：孔德贤[1,2] 李清禄[1] 池毓务[2] 陈国南[2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福州大学化学化工学院食品安全分析与检测教育部重点实验室,福建福州350116

出　　处：《分析测试学报》2013年第11期1283-1288,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21075018);教育部新世纪优秀人才支持计划(NCET-10-0019);福建省教育厅JB类项目(JB09304)

摘　　要：基于6-丙基-2-硫脲嘧啶(PTU)对三联吡啶钌(Ru(bpy)_3^(2+))电化学发光(ECL)有强烈的增敏作用,建立了流动注射电化学发光(FI-ECL)检测PTU的新方法。通过考察影响体系电化学发光的因素(如电压信号类型及电位、缓冲溶液pH值、载液流速及Ru(bPY)_3^(2+)浓度等),得到FI-ECL测定PTU的最佳条件,并提出了可能的反应机理。研究结果表明,在0.1 mol/L pH 12.0的H_3PO_4-NaOH缓冲介质中,控制恒电位为+1.50 V,Ru(bpy)_3^(2+)浓度为1.0×10^(-4)mol/L,载液流速为1.5 mL/min,PTU对Ru(bpy)_3^(2+)的电化学发光具有最大的增敏作用。在此条件下,测得PTU的线性范围为2.0×10^(-7)~1.0×10^(-4)mol/L(r^2=0.998 6),检出限(S/N=3)为1.3×10^(-8)mol/L,对7份浓度均为1.0×10^(-6)mol/L的PTU标准溶液进行测定,所得结果的相对标准偏差为1.2%。该方法简便、快速、灵敏,用于模拟动物饲料中PTU含量的检测,结果满意。Based on the fact that 6-propy-2-thiouracile（PTU） could strongly enhance the electrochemiluminescence（ECL） of tris（2,2’-bipyridine） ruthenium（Ⅱ）（Ru（bpy）2＋3）,a new flow injection analysis method with a homemade electrochemiluminescence（FI-ECL） detector was proposed for the first time.The experimental parameters those may influence the ECL intensity of the Ru（bpy）2＋3/PTU system（such as voltage signal mode and applied potential,pH of buffer solution,flow rate of the carrier solution,and concentration of Ru（bpy）2＋3solution ） were investigated.The optimum conditions for the FI-ECL detection of PTU were obtained and the possible mechanism of the ECL system was suggested.The results showed that in 0.1 mol？L-1 pH 12.0 H3PO4-NaOH buffer solution,when the carrier solution’s flow rate was setted at 1.5 mL？min-1,the strongest ECL intensity enhancement could be realized at potential of ＋1.50 V in the present of 1.0×10-4 mol/L Ru（bpy）2＋3.Under this optimal condition,the linear range of PTU was 2.0×10-7-1.0×10-4 mol/L with a correlation coefficient of 0.998 6 and a detection limit（S/N=3） of 1.3×10-8 mol/L.The relative standard deviation（RSD,n=3） for 1.0×10-6 mol/L PTU standard solution was 1.2%.The method was simple,rapid and sensitive,and was successfully applied in the determination of PTU in spiked animal feeds.

关 键 词：流动注射 电化学发光 6-丙基-2-硫脲嘧啶 三联吡啶钌 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]