基于铁氰化铜的非标记型核酸适配体传感器的构建及其对腺苷的检测  

A Label-free Aptamer Biosensor Based on Copper Hexacyanoferrate for the Determination of Adenosine

作　　者：杨绍明[1] 李红[1] 孙清[1] 李瑞琴[1] 尚培玲 郑龙珍[1] 陈爱喜[1] 

机构地区：[1]华东交通大学基础学院化学化工系,江西南昌330013

出　　处：《分析测试学报》2013年第11期1296-1301,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21065004);江西省教育厅科技项目(GJJ12304);华东交通大学研究生创新资金资助项目(YC2012-X020)

摘　　要：采用电化学沉积法在金电极表面制备了铁氰化铜(CuHCF)氧化还原电化学探针,通过CN^-(CuHCF)和金纳米粒子(GNPs)之间形成Au-CN键的强相互作用力,将GNPs组装到电极表面后,再通过Au-S键将巯基化的腺苷适配体组装到电极表面,构建了高灵敏检测腺苷的非标记型核酸适配体传感器。利用电化学阻抗对传感器的组装构建过程进行监测。用循环伏安法和差分脉冲法考察了该传感器的电化学行为,并探讨了支持电解质和扫速对传感器的影响。在最优实验条件下,该传感器对腺苷在100 fg/mL^50.0 ng/mL范围内呈良好的线性响应,相关系数为0.998,检出限为45.0 fg/mL。Copper hexacyanoferrate（CuHCF） redox probes were fabricated on the gold electrode surface through electrodeposition.Through the strong interaction of Au-CN bond between CN-（CuHCF） and gold nanoparticles（GNPs）,GNPs were assembled on the CuHCF modified electrode.Then,thiolated adenosine aptamers（APT） was assembled on the electrode surface with Au-S bond for the fabrication of highly sensitive label-free aptamer biosensor used for the determination of adenosine（AD）.Electrochemical impedance spectroscopy was used to monitor the self assembly process of the aptamer biosensor.The electrochemical behavior of the aptamer biosensor was studied by cyclic voltammetry（CV） and differential pulse voltammetry（DPV）.Effects of the supporting electrolytes and scan rates on the response of aptamer biosensor were investigated.The biosensor showed a good linear relationship for adenosine in the range of 100 fg/mL-50.0 ng/mL,with a correlation coefficient（r） of 0.998 and a detection limit of 45.0 fg/mL.

关 键 词：铁氰化铜 纳米金 核酸适配体传感器 腺苷 

分 类 号：O657.1[理学—分析化学] Q524[理学—化学]