蚯蚓纤溶酶的亲和层析纯化及部分性质  

Application of Affinity Chromatography for Purification of Fibrinolytic Enzyme from Earthworm

作　　者：林少琴[1] 余萍[1] 兰瑞芳[1] 

机构地区：[1]福建师范大学实验中心,福州350007

出　　处：《药物生物技术》2000年第4期229-232,共4页Pharmaceutical Biotechnology

基　　金：福建省教委资助项目!编号JA980 89

摘　　要：用牛血纤溶酶原Separose4B亲和层析方法从蚯蚓粗提物丙酮粉中分离纯化一种纤维蛋白溶解酶。该酶为糖蛋白 ,含糖量约为 1.43 % ,Mr为 30 0 0 0。实验结果表明此酶具有直接溶解纤维蛋白和激活纤溶酶原的间接溶解纤维蛋白的双重作用 ,并对人血凝块有明显的溶解作用。 1%PMSF对酶有显著的抑制作用 。A fibrinolytic enzyme was isolated and purified from the crude extract of the earthworm by using affinity chromatography on bovine plasminogen sepharose 4B column.It was confirmed to be glycoprotein and the neutral saccharide content of the enzyme is about 1.43%.The molecular weight of the fibrinolytic enzyme is about 30kD as determined by sodium dodecyl sulfate polyacrylamide electrophoresis. The experiment results showed it has strong thrombolytic action on human fibrin and blood clots. The bovine plasminogen can be activated by the fibrinolytic enzyme and it was inhibited by 1% PMSF.The result indicated that enzyme seems to be serine type protease with trypsinlike activities.

关 键 词：蚯蚓纤溶酶 纤溶酶原 亲和层析 纯化 抗血栓药 

分 类 号：R973.2[医药卫生—药品] TQ464.8[医药卫生—药学]