海洋硫酸多糖911抗艾滋病毒作用及其机理研究——体内对猴免疫缺陷病毒(SIV)增殖的影响  

STUDIES OF THE ANTI-AIDS EFFECTS OF MARINE POLYSACCHARIDE DRUG 911 AND ITS RELATED MECHANISMS OF ACTION

作　　者：辛现良[1] 丁华[1] 耿美玉 梁平方[1] 李英霞[1] 管华诗[1] 

机构地区：[1]青岛海洋大学,青岛266003 [2]山东医科大学基础医学院药理学教研室,济南250012

出　　处：《中国海洋药物》2000年第6期4-8,共5页Chinese Journal of Marine Drugs

基　　金：国家海洋"86 3"资助!项目 (编号 819-0 5 -0 2 )

摘　　要：采用体外细胞培养技术和病毒体内感染模型 ,通过 Elisa、定量 PCR、流式细胞术等方法 ,研究了海洋硫酸多糖 911体外对 HIV- 1复制及体内对 SIV增殖的影响 ,并初步探讨其作用机制。结果发现 911可明显抑制HIV- 1对 MT4 细胞的急性感染和 H9细胞的慢性感染 ,其半数有效浓度 ( EC50 )分别为 4 .4 4 mg· L-1和 0 .32mg· L-1;并可明显降低猴血浆中病毒滴度及 RNA拷贝数 ,对血液中 CD4 +细胞具有一定的保护作用 ,同时可升高血液中病毒抗体的含量 ;并可明显抑制病毒逆转录酶活性 ,对 HIV- 1无明显直接灭活作用 ,但可明显干扰 HIV- 1与细胞的吸附 ,半数有效浓度 ( IC50 )为 36.51μg· L-1。提示 911体内外均可抑制艾滋病毒的增殖 ,为高效的艾滋病毒增殖抑制剂 ,其作用机制与干扰病毒与细胞吸附、抑制病毒逆转录酶活性有关。The anti AIDS effects of marine polysaccharide drug 911 both in vitro and in vivo were studied in this paper. The results indicated that 911 inhibited both acute and chronic infection of HIV 1 to MT 4 and H 9 cells with its half effect concentration (EC 50 ) at [HT5,5”] 4.44 mg·L 1 and 0.32mg·L 1 respectively in vitro. While the half toxic concentration (TC 50 ) of 911 to MT 4 cells was 7.78 kg·L 1 and that to H 9 cells 7.47g·L 1 . And the in vivo study showed that oral administration 911 reduced the virus titer and decreased plasma RNA copies in the macaques at doses of 64mg·d 1 and 320mg·d 1 . The protective effect on CD 4 + cells and antibody increasing activity of 911 were also found. Furthermore, 911 significantly interfered the adsorption of HIV to MT 4 cells and suppressed activity of reverse transcriptase. These findings indicated that 911 inhibited the viral replication both in vitro and in vivo by blocking the adsorption of virus to host cells and counteracting the activity of reverse transcriptase and modulating immune function as well.

关 键 词：海洋硫酸多糖911 AIDS HIV-1 SIV 艾滋病 

分 类 号：R512.91[医药卫生—内科学] R978.7[医药卫生—临床医学]