新疆陆地棉抗性生物技术育种的新改进  

New Improvement of Biotechnolgical Breeding for Enhancing Resistance in Gossypium Hirsutum of Xinjiang

作　　者：孙敬三[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《新疆农业大学学报》1999年第4期259-265,共7页Journal of Xinjiang Agricultural University

基　　金：国家"九五"重中之重棉花科技攻关项目!(编号 :95 - 0 0 1- 0 4 - 0 2 )

摘　　要：对棉花生物技术育种的几项重要技术进行了改进 :①建立起一种简易、快捷的陆地棉再生体系 ,结合试管苗扩繁和嫁接技术 ,有效地缩短了培养时间 ,提高了试管苗的成活率。②以绿色荧光蛋白 (GFP)基因为报告基因 ,建立起操作简单、能活体观察、经济、方便的转化体筛选方法。③利用半配合突变体 ,建立了包括单倍体鉴定 ,染色体加倍 ,抗虫性检测等一套快速纯化转基因抗虫棉的程序和方法。The improvement has been made in several important technique of biotechnological breeding of cotton,which includes the following items:①an easy and convenient system of plantlet regeneration,which together with propagation of tube plant and graft technique effectively shortens duration of the culture and increases the surviving ratio;②an economical and convenient method of screening transgenic callus and plantlet in vivo by means of the reporter gene of green fluorescent portein(GFP)gene;and ③ aprocedure of obtanining homozygous quickly by taking advantage of emigamy,including identification of haploid,doubing of chromosome and inspection of resistant insect.

关 键 词：陆地棉 植株再生 生物技术育种 抗生育种 

分 类 号：S562.035.3[农业科学—作物学]