分离培养和血清学试验诊断肺炎支原体早期感染的应用及评价  

Aapplication and evaluation of isolating culture and serological test in the early diagnosis of mycoplasma pneumoniae infection

作　　者：潘庆军[1] 朱学芝 

机构地区：[1]广东医学院附属医院,广东湛江524023 [2]广东粤海饲料集团有限公司,广东湛江524017

出　　处：《检验医学与临床》2013年第22期2956-2957,2959,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81202346);广东省自然科学基金资助项目(S2012040006216);广东省卫生厅基金资助项目(B2012284);湛江市科技攻关项目(2012C3101028)

摘　　要：目的应用并评价肺炎支原体(MP)快速培养法、被动凝集法检测总抗体和酶联免疫吸附试验(ELISA)检测免疫球蛋白M(IgM)抗体在MP感染早期诊断中的价值。方法应用MP快速培养法对该院2009年10月至2010年4月260例急性下呼吸道感染住院惠者的咽拭子标本进行培养,同时用被动凝集法检测患者血清中MP的总抗体和ELISA检测MP的IgM抗体。结果 260例急性下呼吸道感染患者咽拭子MP快速签定培养法培养阳性58例,阳性率22.3%。被动凝集法检测总抗体阳性83例,阳性率31.9%。ELISA检测IgM抗体阳性67例,阳性率25.8%。结论 MP IgM抗体检测试剂因其方便,快捷,可作为优先检测试验。Objective To apply and evaluate the mycoplasma pneumoniae（MP） rapid culture method and the value of passive agglutination detecting total antibody and IgM antibody test in the early diagnosis of MP infection. Methods MP rapid culture reagent was used in the culture of pharyngeal swab specimens in 260 patients that had a- cute lower respiratory tract infections from Oct. 2009 to April. 2010 in our hospital. The total antibodies of MP were detected by passive agglutination and IgM antibody was detected by ELISA. Results 58 of 260 cases accepted MP rapid culture method were positive, and the positive rate was 22.3 ~. There were 83 cases with positive result of pas- sive agglutination method,and the positive rate was 31.9%. There were 67 cases with positive result of IgM antibod y,and the positive rate was 25.8%. Conclusion The MP lgM antibody test is convenient and fast,and can be used as a priority testing.

关 键 词：肺炎支原体 培养法 被动凝集法 酶联免疫吸附试验 

分 类 号：R725.631[医药卫生—儿科]