Effect of Phytohormones on Adventitious Bud Differentiation from Bulb Scales of Oriental Lily Test-tube Plantlets  

植物激素对东方百合试管苗鳞片分化不定芽的影响(英文)

作　　者：王菲彬[1] 王斐[1] 管玲玲[1] 胡凤荣[1] 

机构地区：[1]南京林业大学,江苏南京210037

出　　处：《Agricultural Science & Technology》2013年第10期1365-1368,1382,共5页农业科学与技术（英文版）

基　　金：Supported by Special Fund for the Dominant Subjects Development of the Higher Education Institutions of Jiangsu Province;Science and Technology Innovation Fund of Nanjing Forestry University(X09-120-4)~~

摘　　要：[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.[目的]研究不同激素种类及浓度对试管苗鳞片分化不定芽的影响。[方法]将Tiber、Rodina和Constanta试管苗鳞片在含有不同种类和浓度的激素的培养基中,统计三种东方百合在各处理下的不定芽数。[结果]细胞分裂素对东方百合鳞片分化能力的影响在品种间存在差异,其中6-BA对Tiber和Rodina鳞片分化不定芽的作用效果最好,只是在适宜的浓度水平上因品种不同有所差异。KT对Constanta鳞片分化不定芽的作用效果最好。生长素对3种东方百合试管苗鳞片分化不定芽质量影响差异不明显,只是在分化系数上有所差异。[结论]适合Tiber、Rodina和Constanta试管苗鳞片分化不定芽的培养基分别为MS+0.2 mg/L 6-BA+0.2 mg/L 2,4-D、MS+1.0 mg/L 6-BA+0.2 mg/L IAA和MS+1.0mg/L KT+0.5 mg/L 2,4-D。

关 键 词：Oriental lily Bulb scales of test-tube plantlets PHYTOHORMONE Tissue culture 

分 类 号：S682.29[农业科学—观赏园艺]