In vitro Drug Sensitivity of Eperythrozoon from Mink  

水貂附红细胞体体外药物杀灭试验(英文)

作　　者：高光平[1] 高桂生[1] 史秋梅[1] 张艳英[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《Agricultural Science & Technology》2013年第10期1451-1453,共3页农业科学与技术（英文版）

基　　金：Supported by Fund of Hebei Science and Technology Department(10960408D01)~~

摘　　要：[Objective] This study aimed to test the sensitivity of Eperythrozoon from mink to various drugs in vitro. [Method] The red blood cells isolated from Eperythro- zoon positive mink was cultured in complete medium （70% RPMI-1640 medium and 30% calf serum）, supplemented with bernier, oxytetracycline, trichlorfon, tylosin, imi- docarb, florfenicol, Fuhongjuesha or primaquine phosphate at the working concentra- tions of 12, 24, 36, 48 and 96 μL/ml, incubated at 37.3 ℃, 5% CO2. [Result] Fuhongjuesha was most efficient for killing Eperythrozoon, followed by trichiorfon and primaquine phosphate, but trichlorfon is toxic. Bernier, imidocarb and florfenicol are efficient. [Conclusion] The study provides a scientific reference for clinical treatment of eperythrozoonosis.[目的]研究各种药物对水貂附红细胞体的体外杀灭效果。[方法]以RPMI-1640为基础培养液,加入30%犊牛血清,选用贝尼尔、土霉素、敌百虫、泰乐菌素、咪唑苯脲、氟苯尼考、附红绝杀、磷酸伯氨喹等药物原粉,在37.3℃、5%CO2气体条件下对水貂附红体进行体外药物杀灭试验。[结果]附红绝杀效果最好;敌百虫和磷酸伯氨喹药物次之,但敌百虫毒性较大;贝尼尔、咪唑苯脲、氟苯尼考有效。[结论]该研究为附红细胞体病的临床治疗提供了科学的理论依据。

关 键 词：EPERYTHROZOON MINK In vitro 

分 类 号：S858.92[农业科学—临床兽医学]