泛耐药铜绿假单胞菌的毒力基因检测和同源性分析  

Detection and homology analysis of virulence genes in pandrug-resistant Pseudomonas aeruginosa

作　　者：陈惠玲[1] 盛慧[1] 叶惠芬[1] 杨英为[1] 杨一言[1] 黄小媛[1] 杨银梅[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院检验科,广州510180

出　　处：《中国感染与化疗杂志》2013年第6期469-472,共4页Chinese Journal of Infection and Chemotherapy

基　　金：广东省科技厅资助项目(2011B031800093);广州市医药卫生科技研究基金资助项目(20121A011033)

摘　　要：目的了解毒力基因在泛耐药铜绿假单胞菌中的分布情况,并分析其同源性。方法采用PCR方法检测毒力基因exoU和exoS,用BOX-PCR指纹图谱分析技术进行同源性分析。结果 53株临床分离的泛耐药铜绿假单胞菌,其中40株毒力基因为exoS+/exoU-型,10株为exoU+/exoS-基因型,1株为exoS+/exoU+基因型,2株为exoS-/exo U-基因型。BOX-PCR结果显示,41株exoS+菌株分为24种基因型,11株exoU+菌株分为7种基因型。结论临床分离的泛耐药铜绿假单胞菌,毒力基因的携带率高,BOX-PCR指纹图谱分析技术适合铜绿假单胞菌的同源性分析,可为医院感染的控制与管理提供病原学基因分型资料。Objective To study the prevalence and sequence homology of virulence genes exoU and exoS in 53 strains of pan- drug-resistant Pseudomonas aeruginosa. Methods The virulence genes exoU and exoS were detected by PCR. Sequence homo- logy was analyzed by BOX-PCR. Results Of the 53 clinical isolates of pandrug-resistant Pseudomonas aeruginosa, the exoS ＋ / exoU- genotype was identified in 40 strains, exoU＋/exoS- genotype in 10 strains, exoS ＋/exoU＋ genotype in 1 strain, and exoS-/exoU- genotype in 2 strains. BOX-PCR results showed that 41 exoS ＋ isolates belonged to 24 genotypes, and 11 exoU＋ strains could be grouped into 7 genotypes. Conclusions The prevalence of virulence genes is high in clinical isolates of pandrug-resistant Pseudomonas aeruginosa. BOX-PCR fingerprint analysis combined with sequence homology analysis is help- ful for effective monitoring and control of hospital pandrug-resistant pseudomonas aeruginosa infection.

关 键 词：泛耐药 铜绿假单胞菌 毒力基因 BOX—PCR 同源性 

分 类 号：R378[医药卫生—病原生物学]